mlsp 300 lab report #2-2

During this practical, we will be measuring the absorbance of Bromocresol green combined with albumin using a spectrophotometer. At what wavelength will we make this measurement?Question 3 Select one:a.320 nmb.400 nmc.628 nmd. 595 nme. 511 nm

Which of the following explains why a more concentrated dye solution has a higher absorbance? A. There are more dye molecules in the solution so more isotopes reach the detector. b. There are more isotopes in solution to emit photons. c. There are more dye molecules in solution to emit isotopes. d. There are more isotopes in solution to emit protons. e. There are more dye molecules in the solution to absorb photons.

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A protein’s concentration can be determined spectrophotometrically by mixing it with an indicator called a Bradford reagent and measuring the amount of light absorbed by the protein. Use the plot below to determine the mass of protein (in micrograms, μμg ) in 100.0 mL of the solution if its absorbance is 0.398. A.  52 B.  3.1 C.  2.4 D.  0.52 E.  0.031

Spectrophotometric Determination of Nitrate in Seawaters Explain the relationship between absorbance and concentration Explain the principle behind a UV-Vis spectrophotometer (key components and how it works) Why must the absorbance in this experiment be read at a wavelength of 543 nm?

Force Completion Once started, this test must be completed in one sitting. Do not leave the test before clicking Save and Submit Remaining Time: 36 minutes, 58 seconds. Question Completion Status: A Moving to another question will save this response. Question 9 Which of the following compounds gives an infrared (IR) spectrum with a peak at 1730 cm (strong peak)? ОН ОН 1 2 3 4 2 3 4 A Moving to another question will save this response. MacBook Air OO0 D00 F4 F2 II F8 F3 F5 F6 F7 F9 $ & 2 5 7 8 tA4 #3

how do you relate structure to fluorescence? Give at least three structural characteristics of a fluorescent molecule. (Discuss the presence of functional groups, the effect of substituents and rigidity.)

Please write a clear observation please (NEAT HAND WRITING PLEASE)!!

13.Which of the following is the reason that a more concentrated dye solution absorbs more light than a less concentrated solution?   A. There are fewer molecules of dye in solution that can absorb electrons, meaning more electrons reach the detector, increasing absorbance.   B. There are more molecules of dye in solution that can absorb electrons.   C. There are more molecules of dye in solution that can absorb isotopes.   D. There are more molecules of dye in solution that can absorb photons, meaning fewer photons reach the detector, increasing absorbance.   E. There are fewer molecules of dye in solution that can absorb photons, meaning more photons reach the detector, increasing absorbance.

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P Please don't provide the handwriting solution

Prepare a 1:200 dilution of the 20 mM Coomassie solution to measure the max wavelength—you need 2 ml for the absorbance measurement but the dilution does not matter as long as the absorbance is in the range of 0.2 to 0.6. Set the initial wavelength at 450 nm. Blank the spec with a tube containing 2 ml water. Use the 1000 ul pipette for water because you need to deliver the same amount of water in each tube for the next exercise.   Why is it when the calculations are done for the given values above, the value for ml is 0.01 for coomassie blue?

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Question 9 The basic spectrophotometer is composed of four components. Which component listed below is NOT one of the four? beam splitter monochromator detector O light source