ELISA REPORT

docx

School

Bryant & Stratton College *

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Course

BIOL 120

Subject

Biology

Date

Jan 9, 2024

Type

docx

Pages

2

Uploaded by MagistrateDog4010

Report
ELISA Lab Report Data Table Sample Color Test Result Positive Control Dark Purple Positive Negative Control Light Green Negative Patient A Dark Purple Positive Patient B Light Green Negative Patient C Light Green Negative Patient D Light Purple Weak Positive Patient E Light Green Negative Patient F Dark Purple Positive Questions 1. What basic principles of antibody-mediated immunity are utilized in an ELISA assay? They’re based on the antibodies that are specifically to target that particular antigen and bind them tightly together. 2. How does an ELISA indirectly detect infection by a disease-causing agent? The antibodies are present since the antigen is in the body, which is used to detect the specific antigen. 3. What is the function of the secondary antibody and chromogen in an ELISA? The secondary antibody recognizes and binds to the primary antibody if it is present. The Chromogen links to the secondary antibody if it is present, and it changes the color 4. Why did you perform 3 identical tests for each control and patient sample? To ensure the results are consistent 5. What might cause some positive results to be lighter in color than others? Light color shows that there is few antibodies in the blood that had diseases causing agents. The 6. Describe the disease-causing pathogen for the disease you assayed for, including its mode of transmission to people.
ELISA Lab Report Human immunodeficiency Virus is responsible for causing Acquired Immune Deficiency Syndrome. HIV transmission is: blood, body fluids, breast milk and sex. 7. Write your reaction to the diagnosis of the patients you tested. Were you surprised by the outcome of the test based on the scenario you were provided? How do you think infection could have be prevented in the patients who tested positive? I believe that the infection could have been prevented by using sexual protection and having partners be tested prior to having sexual activity.
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