Lab 10 Submission Sheet

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Holy Family University *

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Biology

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Jan 9, 2024

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Lab 10 Submission Sheet (Combination IP/Online) Laboratory 10: Anatomy of the reproductive system, Gametogenesis and Female Cycles Learning Objectives: 1. Describe the gross and microscopic anatomy of male and female reproductive system structures. 2. Describe oogenesis and spermatogenesis 3. Understand the complexity of male and female reproductive development. 4. Describe the ovarian cycle and uterine cycle and an understanding of the fluctuations in hormone level Laboratory Material List: testis, sperm, ovary, and uterine endometrium slides Activity 1. Gross Anatomy of the Reproductive System Locate the following structures on the reproductive models and any instructor handouts. Testes Scrotum Ejaculatory duct Epididymis Ductus or Vas deferens Urethra Seminal vesicles Prostate gland Bulbourethral glands Ovaries Fallopian tubes Isthmus Ampulla Infundibulum Fimbriae Uterus Cervix Vagina Activity 2. Histology of the Reproductive System 1. Examine slides of the testis. When you view the slide on 100x, the circular seminiferous tubules are visible where sperm are produced. Zoom in on one of these tubules on 400x magnification. The immature sperm are formed from the outside of the tubules to the center. The immature sperm with tails can be faintly

seen in the center. The cells in between the seminiferous tubules are Leydig cells that produce testosterone. Describe the slides below. Please use scientific terminology in the description. i.e., Use the names of cell types and connective tissue names not “it looks purple and blotchy”. Testis 100 X mag Testis 400X mag 2. Observe the sperm slide under the microscope at 400x. The sperm stain a purple color. Describe sperm that you see including the names of the 3 different parts of a sperm. Sperm 400X 3. Observe the layers of the uterine endometrium. Describe the layers and use scientific language including the names of cells and layers of the endometrium. Uterine endometrium 4. Observe the ovary slide at 100x. Find one of the following follicles and oocytes: primordial follicle, primary follicle, or vesicular follicle. Describe the one you found in scientific language. Ovary 100X

Activity 1. Gender Testing of Female Athletes 1. Go to the following Howard Hughes Medical Institute website. Click on Case Studies and do Case Study 1 Sprinter and then Case Study 2 Swimmer. https://media.hhmi.org/biointeractive/click/testing-athletes/case-studies-- sprinter.html 2. What sex does a physical examination determine this athlete to be? Case 1 Case 2 3. What sex does the Barr Body test determine this athlete to be? Case 1 Case 2 4. What sex does the SRY DNA determine this athlete to be? Case 1 Case 2 5. What sex does the testosterone level determine this athlete to be? Case 1 Case 2 6. What is the genetic issue for each of these athletes? (number of chromosomes and what sex chromosomes are present) Case 1 Case 2

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7. What is the abnormal physiology that causes the athletes condition? Case 1 Case 2 8. Do you think these athletes should be able to compete in their sports? (yes/no) Case 1 Case 2 9. Why or why not for the above question? Case 1 Case 2 Activity 2. Reproductive Anatomy and Physiology Questions 1. Explain why a male who takes anabolic body building steroids often begin to exhibit female secondary sex characteristics? (testes shrinking, breast enlargement) What are these anabolic body building steroids? (You do not have to find a specific name just generically what are the steroids) 2. What are the two main hormones in most birth control pills? (Not trade name, just hormone name) 3. How does the vagina get its acidic pH?

4. Find 3 of the differences and similarities between oogenesis and spermatogenesis? Put them in the chart below. Similarities Differences 5. Compare levels of estrogen and progesterone in the first half of the menstrual cycle. (Look at levels of each-use charts in text and slides) estrogen progesterone 6. Compare levels of estrogen and progesterone in the second half of the menstrual cycle. (Look at levels of each-use charts in text and slides) estrogen progesterone

7. Compare levels of FSH and LH just prior to ovulation. FSH LH How to draw in a Word Document: Click on INSERT. Click on SHAPES. Go to Lines and click on the last choice which looks like a “scribble”. Once you do that you should see a black cross. If you left click on the black cross, it turns into a pen icon and will allow you to draw any shape you need. When you release the left click, it stops drawing and you need to start again. If you need to delete a mistake, click on the line, and hit the delete key. 5. Draw in the levels of hormone for LH, FSH, Estrogen and Progesterone in the graph below. Then label which days correspond to the menstrual, proliferative, and secretory phase.

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