CTQ 3

pdf

School

University of Guelph *

*We aren’t endorsed by this school

Course

309

Subject

Biology

Date

Jan 9, 2024

Type

pdf

Pages

3

Uploaded by BarristerHummingbirdMaster241

Report
1 CRITICAL THINKING QUESTIONS 3 (Total: 12 marks) Due October 6 by 6 pm EST 1. Using a probe, you want to determine if a 200 bp CDS exists in the genome of a prokaryotic organism whose genome has not been sequenced. You know the sequence of the CDS you are trying to detect because this CDS exists in E. coli whose genome is sequenced, and because you think that the prokaryotic organism under study might be evolutionarily related to E. coli , the prokaryote might have the same gene. a) What do you think can be used as a template for generating the probe needed to find the gene of interest? (2 marks) You can use the prokaryotic organism as a template for generating a probe, this is due to the fact that we need to detect if our gene of interest is present within the prokaryotic organism, if we see the probe make a signal it is there. b) Note that you want to find out if the CDS with the exact sequence is present in the prokaryote under study and you are not interested in finding related sequences or sequences that have partial similarity but are not identical. In this scenario, do you think making a probe against the 200 bp CDS is better, or should we target either of the UTRs as UTRs are known to be the most unique regions of genes? Explain your answer. (2 marks) Well, we should target the UTRS since we need to know if the 200 bp is entirely present in the prokaryotic. By targeting the UTR of the prokaryotic gene, we can infer any similarities to eukaryotic. While UTR are very unique in organism and can share differing exons, 200 bp can bind nonspecifically, since it can have some similar or un similar genes to bind to. c) You see 4 different signals post-hybridization of the probe. What does this result tell you? (2 marks)
2 That our gene of interest is present within the prokaryotic even if it isn’t a large amount, enough similarities exist to warrant a response from the probe. More signals mean it has detected more complimentary genes of interest. d) You are looking to clone this CDS in a plasmid for further research purposes. You have the CDS on the membrane bound to a probe. Can you purify the DNA from the membrane and use it for cloning? Explain. (2 marks) You can not purify it. Since probes are only used for detection of gene sequences, once the dna is attached to the membrane you can never isolate it. 2. You want to make a 1 liter solution of 200 mM NaCl. The molecular weight of NaCl is 58.44g. a) How much pure NaCl powder will you add in water to make the above solution? (2 marks) 200mM -> 0.2 M N = V * mM (NaCl) N = 1 mL * 0.2 N = 0.2 m = n * 58.44 m= 11.69 b) How can you make the above solution from a 1M stock solution of NaCl? (2 marks) C1v1=C2V2 0.2 (1) = (1) (V2)
3 0.2/1 = V2 V2 = 0.2 L
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
  • Access to all documents
  • Unlimited textbook solutions
  • 24/7 expert homework help

Related Documents

Graded HW 2 (1) (1).docx
Diffusion.pdf
Anatomy Lab Homework 3 (1).pdf
Anatomy Lab Homework 2 (1).docx
Individual Assignment_1.docx
Worksheet Week 7.pdf
Worksheet Questions 5.pdf
Worksheet Questions 4.pdf
CTQ 8.pdf
CTQ 9.pdf
Week 5 lab ap 255.pdf
Derak-Photosynthesis Lab Report Rough Draft.pdf
Recommended textbooks for you
Text book image
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Text book image
Biology: The Dynamic Science (MindTap Course List)
Biology
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Cengage Learning
Text book image
Biology: The Unity and Diversity of Life (MindTap...
Biology
ISBN:9781305073951
Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Publisher:Cengage Learning
Text book image
Biology: The Unity and Diversity of Life (MindTap...
Biology
ISBN:9781337408332
Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Publisher:Cengage Learning
Text book image
Biology (MindTap Course List)
Biology
ISBN:9781337392938
Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher:Cengage Learning
Text book image
Anatomy & Physiology
Biology
ISBN:9781938168130
Author:Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark Womble
Publisher:OpenStax College
SEE MORE TEXTBOOKS
Recommended textbooks for you
Text book image
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Text book image
Biology: The Dynamic Science (MindTap Course List)
Biology
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Cengage Learning
Text book image
Biology: The Unity and Diversity of Life (MindTap...
Biology
ISBN:9781305073951
Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Publisher:Cengage Learning
Text book image
Biology: The Unity and Diversity of Life (MindTap...
Biology
ISBN:9781337408332
Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Publisher:Cengage Learning
Text book image
Biology (MindTap Course List)
Biology
ISBN:9781337392938
Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher:Cengage Learning
Text book image
Anatomy & Physiology
Biology
ISBN:9781938168130
Author:Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark Womble
Publisher:OpenStax College
SEE MORE TEXTBOOKS