chp 13 control of microbe growth worksheet-1

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BIO208 Control of Microbial Growth, Chapter 13 Prior to drawing blood for a blood donation, the nurse will clean the arm with a Betadine solution. This form of antimicrobial control is called __________. A. antisepsis B. disinfection C. sterilization D. decontamination E. degerming To prepare for surgery, the surgical instruments undergo____and the area on the patient undergoes____. A. sterilization, disinfection B. disinfection, sanitization C. sterilization, antisepsis D. sanitization, antisepsis E. decontamination, antisepsis What is the difference between a disinfectant and an antiseptic? A. Disinfectants can be used only on living tissue, antiseptics can be used only on inanimate objects. B. Disinfectants mechanically remove microbes, antiseptics remove microbes with chemicals. C. Disinfectants remove all microbes, antiseptics reduce the microbe load. D. Disinfectants are applied to inanimate surfaces, antiseptics are used on living tissue. A protocol with a long DRT is more effective than a protocol with a short DRT. A. True B. False Once a _____ agent is removed, growth of microbes might resume. A. germicide B. bactericide C. bacteriostatic D. biocide What is the DRT for each protocol? • Tincture of iodine = 85 seconds • Tincture of Zephiran = >120 seconds • Aqueous Zephiran = >>120 seconds • Soap and water = >>>120 seconds Based on the graph , which is the most effective disinfectant? 1

A. Tincture of iodine B. Tincture of Zephiran C. Aqueous Zephiran D. Soap and water Once a _____ agent is removed, growth of microbes might resume. A. germicide B. bactericide C. bacteriostatic D. biocide The shortest length of time required to kill all test microbes at a specified temperature is the thermal death point. A. TRUE B. FALSE According to the graph what is the thermal death time for this culture? A. 60 minutes B. 30 minutes C. 15 minutes D. Cannot be determined Moist heat methods have to operate at higher temperatures and longer exposure times as compared to dry heat methods to kill microbes. A. TRUE B. FALSE Which graph best depicts the effect of placing a bacteria culture at 4°C at time x ? 2

A. a B. b C. c D. d E. e Match each chemical control agent with the correct description. 1. Halogens D 2. Phenolics C 3. Alcohol B 4. Surfactants E 5. Heavy metals A a) Mercury and silver are examples b) A 70% solution is best for denaturing microbial proteins c) Used in Lysol and Listerine d) Chlorine and iodine are examples e) Disrupt cell membranes but only for low-level disinfection 3

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Different disinfectants are soaked on sterile pads that are then placed on a lawn of bacteria. The photo below shows the results after 24 hours of incubation. Rank disinfectants A-F from MOST to LEAST effective. A, B=D=C, E 4

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