Assigment lab10

pdf

School

Rutgers University, Newark *

*We aren’t endorsed by this school

Course

202

Subject

Biology

Date

Jan 9, 2024

Type

pdf

Pages

3

Uploaded by MegaGalaxy12986

Report
Shaniah Idehen LAB 10 WORKSHEET 1. Briefly describe the purpose of working with embryoless seeds. (15 points) The purpose of using embryo-less seeds during this experiment was to effectively demonstrate the enzyme formation that was induced. Without an embryo, we can see that the GA hormone is a contributing factor in enzyme production. 2. GA is a hormone that functions to? (10 points) GA or Gibberellin is a hormone that supports a-amylase, an enzyme that is found in embryo-less seeds. GA supports the synthesis of this enzyme 3. What is the role of Ca2+ ions in this context? (10 points) Once the GA receptor is activated, Ca2+ ions are used to carry those signals as transduction, along with other GA signals. 4. Which groups are your:Positive control,Negative control, and experimental group? (15 points) Positive control: GA with normal seeds Negative control: Water added with normal and embryo-less seeds Experimental group: GA with embryo-less seeds. 5. Using the data provided below which was obtained using 20ul of enzyme sample and incubated for 30seconds in a total volume of 1ml : a. Calculate the UA for all the samples ( 20 points) [GA] Abs 625 0.05 UA=4.8 0.652 0.1 UA=17.0 0.53 0.5 UA=19.6 0.504 1 UA=30.4 0.396
Shaniah Idehen b. Build a semi log graph by plotting log of [GA] on the x-axis and the UAs on the y-axis (15 points) log0.05=-1.3 log0.1=-1 log0.5=-0.3 log1=0 log5=0.7 c. Use this plot as a standard curve to calculate the [GA] in tube #15 which had an absorbance value of 0.5. (15 points) UA=(0.7-Abs625)*Volume of supernatant (ml) / incubation time (min)* enzyme extract volume (ml) Hint: Volume of supernatant= 1ml Incubation time= 0.5 min Enzyme extract volume = 0.02 ml UA1=[(0.7-0.652)*1]/(0.5*0.02)=0.048/0.010=4.8. UA2=[(0.7-0.53)*1]/(0.5*0.02)=0.170/0.010=17.0. UA3=[(0.7-0.504)*1]/(0.5*0.02)=0.196/0.010=19.6. UA4=[(0.7-0.396)*1]/(0.5*0.02)=0.304/0.010=30.4. UA5=[(0.7-0.249)*1]/(0.5*0.02)=0.451/0.010=45.1. 5 UA=45.1 0.249
Shaniah Idehen
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
  • Access to all documents
  • Unlimited textbook solutions
  • 24/7 expert homework help

Related Documents

LEARNING OBJECTIVES CH 20 ANTIMICROBIAL MEDICATIONS 12-29-2021.docx
Copy of Bio report .pdf
CCM Great Swap Data -2.pdf
EXAM_2_REVIEW_Fire_ecology
E10_ Acid Phosphatase Enzyme Assay.pdf
Lab 11.png
Midterm 2 Essay questions Nov 2023.docx
Photosynthesis Virtual Lab .pdf
Chicago Cyanide Murders.pdf
ES_Prjt2_T1_County Population.docx
Copy of Akshay Kamasamudram - 06 HHMI Photosynthesis Student Worksheet.docx
PATHOLOGY FINAL 11-22-2023.pdf
Recommended textbooks for you
Text book image
3-2-1 Code It
Biology
ISBN:9781337660549
Author:GREEN
Publisher:Cengage
Text book image
Comprehensive Medical Terminology
Nursing
ISBN:9781133478850
Author:Jones
Publisher:Cengage
Text book image
Understanding Nutrition (MindTap Course List)
Health & Nutrition
ISBN:9781337392693
Author:Eleanor Noss Whitney, Sharon Rady Rolfes
Publisher:Cengage Learning
Text book image
Case Studies In Health Information Management
Biology
ISBN:9781337676908
Author:SCHNERING
Publisher:Cengage
SEE MORE TEXTBOOKS
Recommended textbooks for you
Text book image
3-2-1 Code It
Biology
ISBN:9781337660549
Author:GREEN
Publisher:Cengage
Text book image
Comprehensive Medical Terminology
Nursing
ISBN:9781133478850
Author:Jones
Publisher:Cengage
Text book image
Understanding Nutrition (MindTap Course List)
Health & Nutrition
ISBN:9781337392693
Author:Eleanor Noss Whitney, Sharon Rady Rolfes
Publisher:Cengage Learning
Text book image
Case Studies In Health Information Management
Biology
ISBN:9781337676908
Author:SCHNERING
Publisher:Cengage
SEE MORE TEXTBOOKS