Lab 2 Macromolecules Worksheet

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Northern Virginia Community College *

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101

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Biology

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Jan 9, 2024

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To submit this assignment, students will complete the Lab Worksheet, then upload their completed document as a DOC or PDF file in Canvas. BIO 101 Lab 02: Chemistry. Introduction to macromolecules Worksheet Name: ____________________________ Section:___________________ Data Analysis and Synthesis Questions: 1. What are the subunits (monomers) found in each of these types of organic compounds: protein:_____________________ starch: _____________________ lipid: _____________________, and _____________________ Table 1. Positive and Negative Controls Reagent Substance Tested Appearance after test Benedict’s Glucose Water Iodine Starch Water Biuret Albumin water Grease Spot Test Oil Water 2. In the Benedict’s test, the Benedict’s reagent tests for simple sugars, e.g. glucose. a. What is the color of the reaction when glucose is present. _________________ b. _____________ will act as a negative control because it does not contain any sugar. 3. In the Iodine test, Iodine tests for starch, a complex carbohydrate. a. The color of the tube when iodine is added to starch is ____________. BIO 101 Lab 02: Macromolecules 10

To submit this assignment, students will complete the Lab Worksheet, then upload their completed document as a DOC or PDF file in Canvas. b. The color of the tube indicated in part (a) above is a ______________ (positive or negative) test result for the presence of complex carbohydrates. 4. In the Biuret test, the Biuret reagent tests for proteins. Describe the change that occurs when Biuret reagent is added to the test tube when proteins are present. 5. An unknown sample has been tested with Biuret and with Benedict’s solution. a. The Biuret test resulted in a blue color. Based on this test result, what can you determine about the molecule(s) present in the unknown sample? b. The Benedict’s test resulted in a blue color. Based on this test result, what can you determine about the molecule(s) present in the unknown sample? 6. The Grease Spot Test tests for lipids or fats. a. The common feature of lipids is that they do not mix with water. Therefore, lipids and fats are called _________________________ b. As mentioned earlier, this test works because the lipids applied to the paper do not evaporate and instead remain between the fibers of the paper reducing the refraction of light. Why does a drop of water not give the same result? Table 2. Predicted Macromolecule Composition: Presence (+) or Absence (-) BIO 101 Lab 02: Macromolecules 11

To submit this assignment, students will complete the Lab Worksheet, then upload their completed document as a DOC or PDF file in Canvas. Food Simple Sugars Starch Protein Lipids Potato Apple Sunflower Seeds Wesson Oil Milk Ground Beef Beans Table 3. Record if the test was Positive (+) or Negative (-) for each food sample Food Benedict’s Iodine Biuret Grease Spot Test Potato Apple Sunflower Seeds Wesson Oil Milk Ground Beef Beans 7. Draw a conclusion for each prediction made. Summarize your results then compare your predictions to the data, and accept or reject your hypothesis. ● Write a conclusion for each test. BIO 101 Lab 02: Macromolecules 12

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To submit this assignment, students will complete the Lab Worksheet, then upload their completed document as a DOC or PDF file in Canvas. ● If your results match the prediction you made for foods that will test positive for that molecule, accept your hypothesis. ● If your results match the prediction you made for the foods that will test positive for that molecule reject your hypothesis. a. Write a conclusion for the test for simple sugars. b. Write a conclusion for the test for starch. c. Write a conclusion for the test for protein. d. Write a conclusion for the test for lipids/fats. BIO 101 Lab 02: Macromolecules 13

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