M4 Module Review

pdf

School

Portage Learning *

*We aren’t endorsed by this school

Course

171

Subject

Biology

Date

Jan 9, 2024

Type

pdf

Pages

Uploaded by MinisterOctopusPerson886

M4: Module 4 Review Due No due date Points 5 Questions 16 Time Limit None Attempt History Attempt Time Score LATEST Attempt 1 6,191 minutes 5out of 5 Score for this quiz: 9 out of 5 Submitted Nov 19 at 2:10pm This attempt took 6,191 minutes. Question 1 0/0 pts components? Your Answer: encourages its expansion What is another name for a liquid growth media, and what are the basic A liquid or solid formulated to support microbial growth, contains essential nutrients to provide the microbe with a source of carbon/energy which Growth media in a liquid form is often referred to as nutrient broth. As the name implies its composition consists primarily of essential ‘ingredients’ such as a rich source of sugars, amino acids and vitamins. Together, a nutrient broth provides the microbe with a source of carbon/energy, which, in turn, encourages its expansion. Question 2 0/0 pts

What is selective media? Your Answer: Suppress growth of unwanted bacteria and encourage growth of desired microbes, often used for cultivation of human pathogens such as Neisseria meningitides Selective media allows for only the growth of certain microbes while restricting the growth of all others. Question 3 0/0 pts What is differential media? Your Answer: Distinguishes between two, often related, microbes grown on the same media Differential media is used to distinguish between two (or more) related microbes. Question 4 0/0 pts What are the requirements of a fastidious microbe? Your Answer: A fastidious microbe is an organism with complex growth requirements such that if absent it will not grow. Enriched medias thus contain these

Correctl! specific and essential nutrients required for the growth of a particular subset of microorganisms. A fastidious microbe is an organism with complex growth requirements such that if absent it will not grow. Enriched medias thus contain these specific and essential nutrients required for the growth of a particular subset of microorganisms. Question 5 0/0 pts What is agar? Your Answer: A gel-like polysaccharide compound used for culturing microbes; extracted from certain red algae In its simplest form agar is liquid growth media that has had a hardening agent added to it. Agar is created when a polysaccharide derived from seaweed (algae) extract is added to ~ growth media. Agar is used to create a solid, smooth surface on which microbes can grow. Question 6 0/0 pts True or False: LB agar is classified as a non-selective, differential media. True False

Your preview ends here

Eager to read complete document? Join bartleby learn and gain access to the full version

Access to all documents
Unlimited textbook solutions
24/7 expert homework help

LB agar is both a non-selective and non-differential media. LB agar is often used to expand unknown microbial population because it encourages microbial growth due to the presence of universal nutrients (non-differential) and absence of restrictive factors (non- selective). Question 7 0/0 pts What are the three forms of hemolysis found on blood agar plates? Your Answer: Alpha (green), Beta (clear zones) and Gamma (white/tan). Microbes capable of alpha hemolysis present with greenish-brown color colonies due to the incomplete (partial) lysis of red blood cells. Beta hemolysis is classified as the capacity of a microbe to completely lyse red blood cells. The resulting lysis presents as a distinct zone of clearing around the growing colony. Gamma hemolysis designates the absence or lack of hemolytic activity and the resulting colonies are often white/tan in color growing on the red background color of the unaffected blood agar plate. Alpha (green), Beta (clear zones) and Gamma (white/tan). Microbes capable of alpha hemolysis present with greenish-brown color colonies due to the incomplete (partial) lysis of red blood cells. Beta hemolysis is classified as the capacity of a microbe to completely lyse red blood cells. The resulting lysis presents as a distinct zone of clearing around the growing colony. Gamma hemolysis designates the absence or lack of hemolytic activity and the resulting colonies are often white/tan in color growing on the red background color of the unaffected blood agar plate.

Question 8 0/0 pts What is the primary purpose of Columbia CNA agar? Your Answer: As it suppresses the growth of Gram-negative bacteria CNA agar is, therefore, used for isolation of Gram-positive microbes. As it suppresses the growth of Gram-negative bacteria CNA agar IS, therefore, used for isolation of Gram-positive microbes. Question 9 0/0 pts Name the type of plate derived from BAP that contains lysed red blood cells. Your Answer: Lysed red blood cells (RBCs) are a primary component in the formulation of Chocolate agar. Lysed red blood cells (RBCs) are a primary component in the formulation of Chocolate agar. Question 10 0/0 pts Define the selective and differential abilities of a MacConkey agar plate. Your Answer:

MacConkey agar is selective in that only Gram-negative microbes will grow on the agar—Gram-positive microbes simply do not grow. MacConkey agar is also considered a differential media as it distinguishes between microbes capable of fermenting lactose (red colonies) and those that are non-fermenters (white/tan colonies). MacConkey agar is selective in that only Gram-negative microbes will grow on the agar—Gram-positive microbes simply do not grow. MacConkey agar is also considered a differential media as it distinguishes between microbes capable of fermenting lactose (red colonies) and those that are non-fermenters (white/tan colonies). Question 11 0/0 pts What colors would you expect to see on an EMB plate containing E. coli? Your Answer: The EMB plate itself is red in color while in the presence of E coli, the growing colonies will take on a distinctive metallic green sheen. The EMB plate itself is red in color while in the presence of E coli, the growing colonies will take on a distinctive metallic green sheen. Note: You are responsible for knowing the color and general properties (selective vs differential) of all of the agar plates ~ described within this module, with a special emphasis on how a particular agar plate aids in identifying select microbes. Question 12 0/0 pts

Your preview ends here

Eager to read complete document? Join bartleby learn and gain access to the full version

Access to all documents
Unlimited textbook solutions
24/7 expert homework help

What is the name of the process of spreading a bacterial culture onto a petri dish? Your Answer: Plating. Plating microbes can be done using a sterile loop, a sterile swab, or a sterilized wire loop. Each device is simply a means of spreading the bacteria, most commonly in a simple back-and-forth motion, across the plate. Plating. Plating microbes can be done using a sterile loop, a sterile swab, or a sterilized wire loop. Each device is simply a means of spreading the bacteria, most commonly in a simple back-and-forth motion, across the plate. Question 13 0/0pts What is the primary advantage of plating a bacterial culture as opposed to having it grow in solution? Your Answer: The primary advantage of plating a bacterial sample onto agar is that cells are held in place. Unlike in a nutrient broth where bacterial cells can multiply but are free to move around in solution, bacteria plated onto agar are fixed in such a way as to support the formation and visualization of colonies.

The primary advantage of plating a bacterial sample onto agar is that cells are held in place. Unlike in a nutrient broth where bacterial cells can multiply but are free to move around in solution, bacteria plated onto agar are fixed in such a way as to support the formation and visualization of colonies. Question 14 0/0pts What is the purpose of the quadrant streak approach? Your Answer: The purpose of this method is to generate an individual colony so that a single (pure) bacterial sample can be picked from the plate. The purpose of this method is to generate an individual colony so that a single (pure) bacterial sample can be picked from the plate. Question 15 0/0 pts In a three-phase dilution gradient, which phase most likely contains individual colonies: phase 1 (P1) or phase 3 (P3)? Your Answer: Individual colonies are most likely going to appear within the phase 3 streaks. You begin with P1 (highest concentration), dilute during P2 and then further dilute the sample in P3. However, it is possible to see individual colonies in P2 if only a small portion of P1 was carried over into P2.

Individual colonies are most likely going to appear within the phase 3 streaks. You begin with P1 (highest concentration), dilute during P2 and then further dilute the sample in P3. However, it is possible to see individual colonies in P2 if only a small portion of P1 was carried over into P2. Question 16 515 pts As a reminder, the questions in this review quiz are a requirement of the course and the best way to prepare for the module exam. Did you complete all questions in their entirety? Your Answer: Yes Quiz Score: 5 out of 5

Your preview ends here

Eager to read complete document? Join bartleby learn and gain access to the full version

Access to all documents
Unlimited textbook solutions
24/7 expert homework help

Related Documents

BIOL368 Lab manual COVID-19 edition Week 1.pdf

BIOL368 Lab manual COVID-19 edition Wk 7 to Wk 11.pdf

MICRO 22 LAB 10 A REPORT.docx

Proprep - Biochemistry - Protein's Three Dimensional Structure - workbook.pdf

Wallace_Week4_Biology.docx

Recommended textbooks for you

Principles Of Pharmacology Med Assist

Biology

ISBN:9781337512442

Author:RICE

Publisher:Cengage

Microbiology for Surgical Technologists (MindTap ...

Biology

ISBN:9781111306663

Author:Margaret Rodriguez, Paul Price

Publisher:Cengage Learning

Comprehensive Medical Terminology

Nursing

ISBN:9781133478850

Author:Jones

Publisher:Cengage

Comprehensive Medical Assisting: Administrative a...

Nursing

ISBN:9781305964792

Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Publisher:Cengage Learning

Surgical Tech For Surgical Tech Pos Care

Health & Nutrition

ISBN:9781337648868

Author:Association

Publisher:Cengage

3-2-1 Code It

Biology

ISBN:9781337660549

Author:GREEN

Publisher:Cengage

SEE MORE TEXTBOOKS

Recommended textbooks for you

Principles Of Pharmacology Med Assist
Biology
ISBN:9781337512442
Author:RICE
Publisher:Cengage
Microbiology for Surgical Technologists (MindTap ...
Biology
ISBN:9781111306663
Author:Margaret Rodriguez, Paul Price
Publisher:Cengage Learning
Comprehensive Medical Terminology
Nursing
ISBN:9781133478850
Author:Jones
Publisher:Cengage
Comprehensive Medical Assisting: Administrative a...
Nursing
ISBN:9781305964792
Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa
Publisher:Cengage Learning
Surgical Tech For Surgical Tech Pos Care
Health & Nutrition
ISBN:9781337648868
Author:Association
Publisher:Cengage
3-2-1 Code It
Biology
ISBN:9781337660549
Author:GREEN
Publisher:Cengage