Mod2 Data Sheet Osmosis in plant cells

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Houston Baptist University *

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1106

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Biology

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Jan 9, 2024

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BIOL 1106 Module 2 Data Sheet Name: Esa Mohammed PS ID: 2220206 Materials: Red onion Elodea Micro forceps Glass slides Cover slips KimWipes Droppers Tally counters Compound microscopes with 4X and 10X objectives Distilled water 15% NaCl and 1M NaCl solutions Gloves Methods: Part I. Red Onion Cells Red onion cells contain a flavonoid pigment called anthocyanin , which gives the onion its purplish color. The same pigment gives petunias their deep purple color. This pigment allows us to visualize what happens to the water content in a cell when presented with solutions of varying osmotic strength. Video on slide preparation: https://www.youtube.com/watch?v=ZW8c_9fGOHw&t=8s . 1. Obtain a wedge of one petal of a red onion. We want to peel the epidermal layer from the purple side of your onion ring. Begin slowly folding the ring in half, bending the purple sides together. Observe the space between the break. You should see a thin layer of membrane connecting your two sides of the onion piece. Gently peel the onion away from this single-cell layer of epidermal cells and lay the membrane directly across the microscope slide. You may need to use micro forceps to peel away a strip of the purple epidermal layer. You only need a small piece. Patience is key! Lay your strip in the center of the slide. You need a flat sample. If it starts curling up, you need to unfurl it gently. As long as you have a section that is flat that you can work with, a little curling on the edge will be fine (just do not try to count cells in that section of the slide). Donna L Pattison, PhD. Department of Biology & Biochemistry, University of Houston

BIOL 1106 Module 2 2. Place a cover slip over the tissue by orienting the cover slip perpendicular to the glass slide and letting it drop over the sample. Sketch or photograph the cells and include in the space below. 3. Prepare a second slide. Add 1 drop of 15% NaCl solution before applying the coverslip. Observe the cells at 40X magnification. Sketch or photograph the cells in the space below every 2 minutes for 6 minutes. Donna L Pattison, PhD. Department of Biology & Biochemistry, University of Houston

BIOL 1106 Module 2 Donna L Pattison, PhD. Department of Biology & Biochemistry, University of Houston

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BIOL 1106 Module 2 4. To quantitate your observations, count the number of plasmolyzed vs. normal cells. You should assess a single field containing approximately 70-100 cells total. # Plasmolyzed cells= 85 Total number of cells= 100 5. Prepare a third slide. Add 1 drop of distilled water before applying the coverslip. Observe the cells at 40X magnification. Sketch or photograph the cells in the space below every 2 minutes for 6 minutes. 6. To quantitate your observations for the cells in the presence of 15% NaCl, count the number of plasmolyzed vs . normal cells. You should assess a single field containing approximately 70- 100 cells total. # Plasmolyzed cells= 85 Total number of cells= 100 Percentage of plasmolyzed cells = # plasmolyzed/total counted Percentage plasmolyzed in 15% NaCl=________85%___ Donna L Pattison, PhD. Department of Biology & Biochemistry, University of Houston

BIOL 1106 Module 2 Part II. Elodea Cells 1. Place one Elodea leaf on a microscope slide and put a cover slip over the leaf. Observe the cells at 4X to get the cells into focus. Switch up to 40X. Sketch or photograph the cells in the space below. 2. Prepare a second slide. Add 1 drops of 1 M NaCl solution before applying the coverslip. Observe the cells at 40X magnification. Sketch or photograph the cells in the space below after 3 minutes. Donna L Pattison, PhD. Department of Biology & Biochemistry, University of Houston

BIOL 1106 Module 2 3. To quantitate your observations, count the number of plasmolyzed vs normal cells. You should assess a single field containing approximately 100 cells total. # Plasmolyzed cells= 75 Total number of cells= 100 Percentage of plasmolyzed cells = # plasmolyzed/total counted Percentage plasmolyzed in 1M NaCl=____75%_______ 4. Prepare a third slide. Add 1 drop of distilled water before applying the coverslip. Observe the cells at 40X magnification and sketch or photograph the cells after 3 minutes. Donna L Pattison, PhD. Department of Biology & Biochemistry, University of Houston

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BIOL 1106 Module 2 Donna L Pattison, PhD. Department of Biology & Biochemistry, University of Houston

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