08PBL - 2023 (2)

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3121

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Biology

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Jan 9, 2024

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ANSC 3121: Animal Genetics Name: Shayla Roman _____________________ Due Date: November. 8 th 2023 Lab Assignment #8 1. From the lab activity, which samples are BLAD carriers and BLAD-free? Explain how did you identify them. a. BLAD carriers are imported bulls, lanes 3.4.5 are BLAD free, lanes 7 and 8 are BLAD carriers because enzyme HaeIII cut into three pieces. 2. Why did we not have a clear read in the BLAD carrier when using Sanger sequencing? a. When using Sanger sequencing, not having a clear read in BLAD is due to poor sample quality. 3. Results from a single locus probe DNA fingerprint analysis for a man and woman and their four children are shown in the autoradiograph below. Which child is least likely to be the biological offspring of this couple? The child least likely to be the biological offspring of this couple is child 2 because it looks like the mother contributed two bands to the child while none with the father. 4. The diagram below shows an autoradiograph of a DNA sequencing gel. Write the 5' to 3' sequence of the template strand based on the pattern in this gel. 5’ CAACTGGTCCAT 3’ 5. Why can’t we use PCR or Sanger sequencing straight away into an RNA-virus? What should we do in order to use this type of tool in RNA viruses? a. RNA virus has RNA instead of DNA as genetic material so they cannot be sequenced by Sanger or PCR, to use this tool in RNA viruses DNA needs to be converted into cDNA.

6. When should we use PCR or Sanger Sequencing? Justify your answer a. For microssatelites in a paternity test i. We should use PCR because it amplifies the target sites on specific DNA regions. This helps to further analyze patterns in finding out the biological father. b. For a known mutation with no specific restriction enzyme i. Sanger should be used because it determines the exact order of nucleotides in DNA. c. For searching for an specific sequence in the Y chromosome, for sexing a young individual. i. PCR should be used as it is able to amplify the Y chromosome specific to males along with gel electrophoresis. d. For a single point mutation that we have a restriction enzyme. i. PCR should be used because it will amplify the DNA region where the mutation is and then use a restricting enzyme to cut PCR into smaller fragments. e. For searching for a unknown mutation i. To identify unknown mutation Sanger sequencing should be used to show mutations in DNA region.

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