#1 Lab Skills WS (8-30)

docx

School

Santa Clara University *

*We aren’t endorsed by this school

Course

030

Subject

Biology

Date

Apr 3, 2024

Type

docx

Pages

Uploaded by MagistrateWater32378

Names: Mahika Tyagi and Andrea Wat TA: Hallie Group Number: 8 Lab Assignment Ecosystems of the Homewood Campus: Part I (Skills Review) Due at the end of today’s lab 100 points possible Notes:  Work with your lab partner(s) and hand in a single copy of this assignment. If you like, you can post a copy in your Canvas Lab Group homepage so you can all edit it at the same time.  Please include your Group number in the name of your assignment file.  Please type all of your answers in red or purple ink to make it easier for your TA to grade.  Please upload your completed document as a Canvas Assignment submission as a .doc or .pdf file (and not as a .pages file). QUESTIONS ON PART A 1. Practice with Small-Volume Micropipettor a. Take a photo of your micropipettor when it is set for 4uL. Paste the photo here: b. What is the maximum volume this particular micropipettor can measure? How do you know? Page 1 of 4 The maximum volume of this micropipette is 10 microliters. We know because the label lists the range of the pipettor from 0.5 – 10.00 microliters. Additionally, the knob pushes some resistance upon its user if turned past the 10-microliter mark.

c. After you load the tubes into the centrifuge, but before you spin them, take a photo. Paste the photo here. d. W h y is it important to balance the tubes in the centrifuge? Use “speed” in your answer. e. W hat was your result in step 8 of “Practice with Small-Volume Micropipettor”? List one reason that a student might have result “b”. (10 points) Our result was that the tip was filled and there was no solution left inside the centrifuge tubes in all three (A, B, and C). One reason that a student might have had some liquid left in the centrifuge tubes is because as they were taking liquid from the various solutions (1,2,3,4), they went to the second stop instead of the first stop, thus causing a higher intake of solution than intended. 2. Practice with Large-Volume Micropipettor a. What was your result in step 4? Page 2 of 4 It is importance to balance the tubes in the centrifuge to avoid breaking the rotor of the centrifuge when it reaches its maximum speed. Our result was that the tip was filled and there was no solution left in the centrifuge tube, thus indicating an accuracy in pipette measurements and procedure.

b. Why should you not lay the micropipettor down on the bench while there is fluid in the tip? QUESTIONS ON PARTS B AND C 3. How does the K-12 derived E. coli strain we are using in lab differ from other E. coli strains? (10 points) 4. Suppose you forget to flame the spreader before you use it. How might this affect the results of your experiment? (10 points) 5. In the Streaking exercise, you are directed to overlap the beginning of your second streak with the first streak. What would happen if you failed to do this? (10 points) Page 3 of 4 You should not lay the micropipettor down on the bench horizontally while there is liquid in the tip because the liquid will travel up and into the micropipettor’s functional parts and damage the intake and release ability of the pipette. The K-12 derived E. coli strain differs from the typical E. coli strains in that it is ineffective in colonizing in the human gut and lacks the cell-surface “O antigen” that is responsible in the virulence of typical E. coli cells. The primary reason for flaming the spreader is to kill off and remove any pre-existing bacteria that may already be present on the spreader before exposing it to the E. coli cultures. Failing to flame this spreader may result in the presence of other bacteria on the growth culture plate and will, thus, negatively impact the accuracy of the experiment and prevent any accurate observation of E. coli growth on the plate (confounding bacteria will make it nearly impossible to observe the cultures of E. coli ). Additionally, the amount of E.coli would be uncontrollable and the streaks would be unobservable. If there was no overlap in between the second and first streak then the growth of the colonies in the second streak would be virtually nonexistent and the bacteria would be unable to spread from that initial streak into the pattern of the second streak.

Your preview ends here

Eager to read complete document? Join bartleby learn and gain access to the full version

Access to all documents
Unlimited textbook solutions
24/7 expert homework help

QUESTION ON PART D 6. For each tree you visit today, decide what your group thinks are its two most identifiable characteristics. Try to pick characteristics that might apply to other trees of the same species, not just the one tree you are viewing today. (30 points) Page 4 of 4

Related Documents

Final Project BIO 330.docx

Photosynthesis_Cell+Respiration+Lab_rev 1.pdf

Milestone 1 - BIO 330.docx

Dichotomous+Key+Assignment.pdf

BIOS255 Week 4 Lymphatic System Worksheet.docx

What is Life Week 2 Worksheet - FS21.docx

206 Nervous System Worksheet Key.docx

Food+Webs+assignment.pdf

Milestone two SCI 333 local waste stream description.docx

Urinary_System

2013 Exam 4-1.doc

2011 Exam 4-1.doc

Recommended textbooks for you

Case Studies In Health Information Management

Biology

ISBN:9781337676908

Author:SCHNERING

Publisher:Cengage

Essentials Health Info Management Principles/Prac...

Health & Nutrition

ISBN:9780357191651

Author:Bowie

Publisher:Cengage

Comprehensive Medical Assisting: Administrative a...

Nursing

ISBN:9781305964792

Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Publisher:Cengage Learning

Intro To Health Care

Health & Nutrition

ISBN:9781337338295

Author:Mitchell

Publisher:Cengage

3-2-1 Code It

Biology

ISBN:9781337660549

Author:GREEN

Publisher:Cengage

Understanding Health Insurance: A Guide to Billin...

Health & Nutrition

ISBN:9781337679480

Author:GREEN

Publisher:Cengage

SEE MORE TEXTBOOKS

Recommended textbooks for you

Case Studies In Health Information Management
Biology
ISBN:9781337676908
Author:SCHNERING
Publisher:Cengage
Essentials Health Info Management Principles/Prac...
Health & Nutrition
ISBN:9780357191651
Author:Bowie
Publisher:Cengage
Comprehensive Medical Assisting: Administrative a...
Nursing
ISBN:9781305964792
Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa
Publisher:Cengage Learning
Intro To Health Care
Health & Nutrition
ISBN:9781337338295
Author:Mitchell
Publisher:Cengage
3-2-1 Code It
Biology
ISBN:9781337660549
Author:GREEN
Publisher:Cengage
Understanding Health Insurance: A Guide to Billin...
Health & Nutrition
ISBN:9781337679480
Author:GREEN
Publisher:Cengage