Post-Lab 4 Assignment

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Biology

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Apr 3, 2024

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Post-Lab 4 Assignment 1) What are the ( AMY1A ) gene copy numbers for your two DNA samples as calculated from your ImageJ and Excel analysis? Include the table from the Excel file that you used to calculate their gene copy number. (3 marks) As calculated from ImageJ and the Excel analysis, my AMY1A gene copy number is 6 for my first DNA sample and 5 for my second. 2) In lab 2, you generated bar graphs connecting amylase Concentration to self-reported starch consumption using salivary amylase concentrations from labmates. Similarly, gene copy number for AMY1A was collected and recorded from you and your lab mates to provide you a table of AMY1A gene copy number (average of the two DNA lanes) of each of your lab mates. Calculate the correlation coefficient (r), for the complete data set (you and your labmates). Instructions on how to calculate the correlation coefficient can be found in Excel Tutorial #1 (p. 102) on Avenue. Add a caption below your figure. For publication quality figures, which should be created for your formal lab report, titles are not typically placed above the graph, but rather in the caption itself. Your caption should also include the sample size and the value for the correlation coefficient. (10 marks)
3) What can you determine from the ‘r’ value about the relationship between gene copy number and amylase enzyme concentration? (2 marks) The correlation coefficient was determined to be 0. 2087616 . This indicates a weak positive correlation and suggests that the relationship between gene copy number and amylase enzyme concentration is a weak positive relationship. This data suggests that while there is some correlation between the increase in salivary amylase concentration and the number of amylase gene copies, the relationship is not direct. Consequently, the increase occurs in a manner that is inconsistent and unreliable. 4) Reflecting on self-reported starch levels, AMY1A gene copy numbers, and salivary amylase concentrations, is there an association between gene evolution/diet, gene copy number, and amylase enzyme production? (2 marks) Reflecting on self-reported starch levels, AMY1A gene copy numbers and salivary amylase concentrations, there is a slight association between gene evolution/diet, gene copy number and amylase enzyme production. My self-reported starch level was stated to be moderate Figure 1.1 – The Dependence of Salivary Amylase Concentration on Amylase Gene Copy Number The graph above depicts the salivary amylase concentration of a group based on their respective amylase gene copy numbers. The sample size is 20 students. The correlation coefficient (r) is 0.2087616.
(gene copy number projected to be in between the two means of 5.44 and 6.72) due to my cultural and ancestral diet from India (where in there is consumption of at least two sources of carbs). This was reflected in my observed gene copy number of 6. However, the reason for there being a “slight” association was due to the fact that there was a weak positive correlation (r value of 0.21) between amylase gene copy number and salivary amylase. The diet in one’s household is believed to have some impact on their number of AMY1A gene copies, but this influence is considered moderate rather than substantial. From the positive correlation, it suggests that the more starch consumed will lead to a higher amylase gene copy number which then results in one having higher amylase concentration. Grade: /17
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