Week 7 Forensic Entomology 2021 VIRTUAL

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University of Nebraska, Lincoln *

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120L

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Biology

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Apr 3, 2024

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Introduction to Forensic Science FORS 120L, Fall 2021 Week 7: Forensic Entomology and Biological Evidence (Virtual Lab) The lab consists of two parts, entomology and Pollen. Upload assignments from Entomology Overview : Insects infesting habitats, spreading disease, contaminating food products, and helping to determine time of death are some aspects of Entomology that could wind up in court. In most cases, forensic experts would be used in court to testify on Entomological issues. However, the collection of the information and evidence is often done by forensic scientists working as crime scene investigators or lab scientists. Insect Biology, Insect Identification, and Forensic Entomology are core courses for the Forensic Science Degree CSI Track at the University of Nebraska-Lincoln. This lab is designed to give all students a basic introduction to insects as forensic evidence. Insects have six legs, usually two pair of wings except for flies which have one pair, three body regions (head, thorax, abdomen), and often compound eyes.
Flies are one of the most common insects to visit a dead body. Larvae of flies, often called maggots, have distinguishing anatomical features.
The spiracles (breathing holes on the rear of a maggot or on the sides of adult insects) and other features can help to identify flies and other insects. See https://shire.science.uq.edu.au/bb/parasitology/maggots/maggot.html for an interactive maggot
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identification key. During lab you will learn to identify insect body parts and anatomical features, and you will learn to use a dichotomous key to help identify insects. Erin Bauer, an Entomologist with the UNL Entomology Department, conducts the labs (this semester done virtually). In this virtual lab you will examine various maggots. You will also learn to use a dichotomous key to identify other forensically important insects. Reading Assignments (all linked in Canvas): 1. Erin Bauer, Crime Fighting Insects. (PPT lecture notes) 2. Dichotomous Keys 3. Fly Information Sheets (University of Florida) 4. Forensic Entomology Collection Manual. Helene LeBlanc Lab Assignments
1. Use a dichotomous key to identify insects in the photos provided to you below. A dichotomous key is a tool that can help you identity organisms based primarily upon physical characteristics. Dichotomous keys consist of a series of choices that lead to the correct identification. Several keys are available in Canvas to help you. You only need to identify to Order. A. This insect is in the Order ______________________________________ Chewing mouthparts Hardened outer wings Chewing mouthparts Leather-like outer wings
B. This insect is in the Order ___________________________________________ C. This insect is in the Order ____________________________________________ Siphoning mouthparts Scaly wings Number of wings is important
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D. This insect is in the Order ______________________________________ E. This insect is in the Order __________________________________________ F. This insect is in the Order __________________________________________ Fuzzy Four wings Leather-like front wings Walking/running legs
3. View the following fly photos. Describe the physical characteristics (i.e. color and shape of body and eyes, hairs, mouthparts, etc.) that could help identify the species of fly. Identify them by common name (i.e. house fly, stable fly, hairy maggot blow fly, etc.) See the fly information sheets in Canvas to help with identification. A. What characteristics could you use to ID this fly? What is its common name? B. What characteristics could you use to ID this fly? What is its common name? 4. Look at the following microscope photo of a maggot’s spiracles. Identify the specimen to Order and Family (Sarcophagidae or Calliphoridae), including its instar stage. Then, explain how the spiracles help you determine this information.
A. What is the Order and Family of this maggot? What instar is it in? Describe how the spiracles help you determine its identification. 5. Two points extra credit . What is the common name of this arthropod? Identify to Kingdom, Phylum, Class, and Order. ____________________________________________________ Here are links to insect reference libraries if Google doesn’t work for you: https://bugguide.net/node/view/15740 and https://www.insectidentification.org/
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Pollen and Botany Some other biological evidence can be found in areas of forensic medicine, anthropology and DNA, pollen analysis, forensic botany, forensic agronomy, and forensic wildlife. Pollen analysis is a course offered in the UNL forensic science degree program. Pollen analysis can help determine where a person had been during a given time frame. Dr. Karl Reinhard teaches pollen analysis and Taphonomy. In the second part of this lab you will learn to identify physical features of pollen grains that can be used as points of identification. Dichotomous keys are used in the identification of pollen grains. Pollen comes from within the plant. How many different grains can you name?
Can you identify a pollen grain that came from a tree? Reading Assignments: 1. Dr. Vaughn Bryant, Forensic Palynology: Why It Works. http://projects.nfstc.org/trace/2009/presentations/3-bryant-palynology1.pdf . 2. 3-bryant-palonology2 3. Forensic Botany as a Useful Tool Lab Assignments: a. www.eScienceLabs.com . > Log-in > student > University of Nebraska Lincoln Forensics > 2nd Edition Introductory Biology Version 3 > Plant Reproduction. Review the content. You do not have to do any of the experiments. Test questions will be taken from the content
b. www.eScienceLabs.com . > Log-in > student > University of Nebraska Lincoln Forensics > 2nd Edition Introductory Biology Version 3 > Taxonomy. Review the content. Take the quiz. Test questions can come from the quiz. Do experiment
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