Study Guide Exercise 25_Antimicrobial Susceptibility copy

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Dallas Colleges *

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2402

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Biology

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Apr 3, 2024

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Study Guide Exercise 25. Antimicrobial Susceptibility Exercise 25. Antimicrobial Susceptibility Background Define antimicrobial: a general term for something that kills or inhibits microorganisms. Differences between broad spectrum and narrow spectrum drug: broad spectrum work well with all the bacteria tested. Narrow spectrum antimicrobials only work against one organism or group of organisms. Examples of broad-spectrum drug: ciproflofloxacin, tetra-cyline, and trimethoprim. Examples of narrow spectrum drug: penicillin against gram positive, polymyxin B against gram negative, and vancomycin If an antibiotic kills a bacterium, then the organism is _ sensitive _. If the antibiotic is ineffective at killing the antibiotic then the organism is _ resistant _ to it. The __ Kirby-Bauer method __ is widely used for testing the sensitivity of bacteria to antimicrobials. What type of medium was used in the method? Mueller-Hinton agar What is a zone of inhibition, and why should it be measured? If the antimicrobial inhibits the growth of the organism, a clear, circular area appears around the disc (concentration of antimicrobials). The measurements of the zones are then compared to standard to determine if the bacteria are sensitive or resistant to it, or if the measurement is between those numbers (intermediate).
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