COVID Pt B

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National University College *

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3810

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Biology

Date

Dec 6, 2023

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2

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DeAndre Borders 25 November 2023 BIO 3810 Georgia State University COVID Mini-Result Part B Table 3, ELISA Spectrophotometry of Samples and Interpretation: Upon acquiring the mean optical density values for every one of the ten samples, the background adjustments factor (BAF) was deducted from every value. Every sample was found to contain SARS-CoV-2 (positive), negative, or borderline using the usual negative OD value range ( 0.18), positive OD value range ( 0.22), and borderline OD value range (0.18-0.22). Patient Average OD (nm) Average OD- BAF (nm) Results Sample 1 0.28 0.216667 Borderline Sample 2 0.685 0.621667 Positive Sample 3 0.16 0.096667 Negative Sample 4 0.20 0.136667 Negative Sample 5 0.80 0.736667 Positive Sample 6 0.57 0.506667 Positive Sample 7 0.745 0.681667 Positive Sample 8 0.67 0.606667 Positive Sample 9 0.145 0.081667 Negative Sample 10 0.93 0.866667 Positive Figure 3, Microneutralization Assay Results: If the hue is light blue, it indicates a virus infection (-), whereas deep blue indicates cell development and no viral infection (+). Samples A
COVID Mini-Result Part B 2 and B exhibit different neutralizing titers: 1:16 and 1:32, respectively. Sample A's titer is lower than Sample B's. Sample B had superior infection-blocking efficacy. Calculations for Table 3: ????𝑎?? ??𝑆 ?? 𝑁??𝑎???? ??????? (𝑥𝑁?) = 0.16333 ?𝑎???????? ????????? 𝐹𝑎???? (??𝐹) = 0.16333 − 0.10 = 0.633 Questions: 1. Based on the results of column 7, are the results from column 1 to 6 reliable or not? Explain. Yes, because the assay displays light blue cells indicating no virus and thus no antibodies in the serum; however, the deep blue cells indicate infection present and a clear presence of antibodies in serum. 2. Compare sample A and sample B. Which one had a better neutralizing effect against the virus? Sample B because it has a higher neutralization titer at 1:64. 3. Determine the highest dilution at which the 3 repeated wells are all (-) for Samples A and B. Sample A: 1:64 Sample B: 1:128 4. The neutralization titer is expressed as the reciprocal of the highest dilution that exhibited total neutralization of viral infection of the cells. (For example, if the highest dilution is 1:8; then neutralization titer is 8). Calculate the neutralization titers for Sample A and Sample B. Sample A: 16 Sample B: 32
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