fleacide

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School

National University College *

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Course

3810

Subject

Biology

Date

Dec 6, 2023

Type

pdf

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2

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BIOL 3800 Or. Cruz Fleacide Mysteryll : Rk . Iy D B P P g Tk AR T t G QTN B YUY, G ema i ! ‘:‘;‘P \é‘“ N e j{ e % e & 'Mx"."" 5 0% N | TSI O £A LY 107" G ¥ Part L. Read the following scenario: A 4 year old girl is brought into the Medical Examiner’s office. You read the following report from the hospital: At 10 am, the mother returns from the store to find the girl vomiting, not sleepmg well and lethargic. Mother puts the girl to bed. Ten minutes later, she noticed that the glrl’s breathing became irregular and slow. She tried to wake her daughter but was unable to do so. The child became comatose. At noon, the girl was admitted to the hospltal with not heartbeat or spontaneous breathing. A police report states the following: The parents discovered that the girl had given her dog a bath using Fleacide. According to the label on the container, Fleacide is an insecticide made of some plant materials and appropriate for external use on animals. Ingredients from label: PERMETHRIN N-OCTYL BICYCLOHEPTENE DICARBOXIMIDE ~——COCONUT OIL —METHOPRENE _ ROTENONE Answer the following questions: 1. What could have been in the flea dip that killed the girl? = QDW\GY\B 2. How could a product that is normally harmless to humans and pets have killed the girl? ({W\LJ{J 3. Also make a quick note of what you learned about any of these chemicals and questions you i yarts B iomON 51 (g (NADH oRdase) sesiEe-S) Part I1. Autopsy report The girl died within two hours of first vomiting. Immediate cause of death was hypoxia (lack of oxygen). Tissue sections from the kidneys, lungs, thymus, and heart show massive cell death. Staining with cellular dyes indicates the mitochondria within the affected tissues were damaged.
BIOL 3800 Or. Cruz Answer the following questions: 1. What function of the cell did the Fleacide effect? - a,wob\ C ‘z E—Sp (fl.\% Part III. ATP analysis A more detailed analysis of the cells from the girl’s heart showed that ATP levels were reduced in the mitochondria. ATP levels in the cytoplasm of these cells however were normal. Acetyl- Coenzyme A levels were normal. - —_— . Answer the following: fifl(? ¢ d 1. What cellular process(es) was impaired by the Fleacide? Zb'r[/ Part IV. Subcellular analysis Using a new chromatographic technology developed late last year, you are able to determine the levels of various subcellular components in the heart cells. Key highlights of the report are listed below: Metabolite Autopsy Finding ~ Nommal Levels Glucose 102 pymol 100 pmadl Pyruvate 23 umal 25 pmol NAD+ 6 pmol 75 pmol NADH © 383 umol 50 pumol Answer the following: 1. Given this new information, what specific cellular process do yqu think was affected by the Fleacide? Why? @10, WA O (glgls were dvxectH 2. Some health food stores sell supplements containing NAD+. Would this have saved her? Wh - \ b( a add gnl) or why not? Nox Was (o due B the pronavie heiPore Thard w 3. Would artificial respiration or oxygenation have saved her? Why or why not? Exercise N fifi' Dt ~Orugen word ol up o g wilh BT W Oxygen nut peesprng e gleckons. e volecuar Ouygp) WOUR Oulah up il Reone damaging o 40t el CPRNRY Wing e mivchondny. | |
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