MCB2010C_Lab4Report

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Tallahassee Community College *

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2010

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Biology

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Feb 20, 2024

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Name(s) : Nicole Linebaugh Section: 2360 Lec/Lab LAB 4 REPORT : BASIC BACTERIAL CULTURING METHODS & ISOLATION OF PURE BACTERIAL CULTURES & PREPARING A STREAK PLATE & PREPARING A SPREAD PLATE 1. Evaluation of streak plate: Attach at least 1 digital image of your streak plate indicating the distribution of the colonies. Is there evidence of contamination among the colonies on your streak plate? No Are you satisfied with your streak plate? No If not, how can you improve your streak-plate technique? I could improve my streak plate by remembering to sterilize an inoculating loop or needle. 2. 3. Evaluation of spread plate: Attach at least 1 digital image of your spread plate indicating the distribution of the colonies. Is there evidence of contamination among the colonies on your spread plate? No Are you satisfied with your spread plate? Yes. If not, how can you

improve your spread-plate technique? 4. Compare the results of your streak and spread plates. Which method achieved the best separation of colonies? The results of my streak plate had more colonies and microbes growing than my spread plate. The method to achieve the best separation of colonies would be the streak plate because, in the streak plate, the colonies are diluted progressively and spread apart with each streak for more isolation. 5. In regard to bacterial growth on solid media, define the term “colony” scientifically. “A colony is a population of cells arising from a single cell or spore from a group of attached cells; Often called a colony-forming unit (CFU).” Tortora, Funke & Case. Microbiology: An Introduction, 13th Ed., 2019: Pearson. 6. Characteristic features of bacterial growth in culture can be used for differentiation of bacterial species. Describe your colonies appeared on your streak plate:

a. How many types of colonies? Two b. Form of colonies? Circular c. Pigmentation of colonies? White/pink d. Margin of colonies? Smooth, entire and lobate. e. Elevation of colonies? Plateau. f. Appearance of colony surface? Round . 7. What advantages do the streak-plate method have over the spread-plate method? Some of the advantages of a streak-plate method are quicker, takes less culture, and the results of a streak plate are individual colony-forming units and isolates for pure cultures whereas a spread-plate method does not. 8. Why do you need to use sterile inoculating loop or re-sterilize the inoculating loop between the 1 st and the 2 nd streaks and between the 2 nd and 3 rd streaks when doing a streak plate? You would need to sterile the inoculating loop or re-sterilize the inoculating loop between the 1 st and the 2 nd streaks and between the 2 nd and 3 rd streaks when doing a streak plate because each section of the streak plate is diluting the sample. 9. If you transfer material from an area of confluent growth on a streak plate to a tube of nutrient broth, can you be sure that the resulting broth culture is a pure culture? Why or why not? No, once taken from the plate air may enter the tube or loop and contaminate. 10. How can you make sure that an isolated colony on a streak or spread plate is really a pure culture? Apply aseptic techniques and take one of the colonies from the streak plate and streak another with that one colony. The results of black and blue is not a pure culture. 11. Spread plate technique is most successful if the concentration of bacteria in the broth mixture is LOW /HIGH (underline one).

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