Lab 10- Identification Of An Unknown Bacterial Culture

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Tallahassee Community College *

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2010

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Biology

Date

Feb 20, 2024

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docx

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Lab 10: Identification Of An Unknown Bacterial Culture In the laboratory experiment by performed a gram stain on Wednesday, November 15th, which allowed us to identify whether our microbe was gram-stain. Upon completion of the gram stain procedure, the microbe exhibited gram-positive characteristics. Next, it was imperative for us to ascertain the shape of the microbe and determine whether it was rod-shaped or cocci. Through careful examination under a light microscope, the microbe displayed a cocci shape and formed clusters. This information enabled to proceed with either utilizing the Gram-Positive Flow Chart or conducting biochemical testing. We opted to perform the Catalase test based on these findings. Subsequently, the Catalase test was conducted, and the microbe produced bubbles, indicating a positive result. This confirmed that our bacterium belonged to the Staphylococci genus. To further narrow down the specific strain of Staphylococci possessed, the Mannitol Salt Agar test to observe if fermentation occurred. Following incubation and careful observation, the conclusion was that the bacterium in question was indeed Staphylococcus Aureus Results from Elise LaCombe in Group #1

Sources: Leboffe, M.J., and Pierce, B.E. (2011). A photographic atlas for the microbiology laboratory (4th Ed.). Morton Publishing.

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