Lab 4 Answer Sheet 1

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Purdue University *

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UNIT 8

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Biology

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Feb 20, 2024

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Save your completed answer sheet as a PDF document and upload to Brightspace. Paste a picture of your plates. (2 points) Record the zone of inhibition and interpret the results for your organism here. Use the zone diameter interpretive chart in your lab’s background to determine resistance or susceptibility. Which bacterial culture did you have? ___e coli_____ Table (6 points) Section Antimicrobial Agent Zone of Inhibition (mm) Resistant or Susceptible A Gentamicin 22 mm Resistant B Novobiocin 0 mm Susceptible C Penicillin 0 mm Susceptible Answer the post-lab questions in the table. 1. (2 points) The size is 2.6 cm which would be 26 mm

2. (2 points) It is susceptible because it has to be greater than 17mm and the zone of inhibition is 26mm 3. (2 points) It would be resistant because 26mm is less than 28mm 4. (2 points) You would prescribe the patient vancomycin cause it is susceptible and penicillin is resistant. You wouldn’t want to prescribe your patient in a medication that is resistant because it would not work 5. (6 points) Penicillin G- prevent the synthesis of peptidoglycan Novobiocin- bind to the 30S ribosomal subunit and cause misreading of the mRNA during translatio Gentamicin- inhibit an enzyme in the dna replication process in some gram positive bacteria 6. (2 points) penicillin because it always goes after stronger infections

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