lab1exam.BIO251

Name _______________________ Lab Exam I BIOL 251 Multiple choice. Select the best answer for the questions below. Note: some choices may be used more than once. a) 45% b) 34% c) 32 μμg (pg) d) 0.9-1.1 e) 14-16 gm% (average 15g%) 1. Average Hb concentration in 100ml of whole blood 2. A normal value you would expect from the Tallquist method 3. Average ratio of packed cell volume to whole blood volume 4. Normal mass of hemoglobin in one red blood cell 5. MCH 6. A weight/volume ratio WITHIN the red blood cell 7. Normal color index Multiple choice. Select the best answer for the questions below. 8. Consider iron deficiency anemia : The MCV of the cell is _1_ and the MCH __2__ and the MCHC is __3_. 1 st 2 nd 3 rd A Stays the same Increased decreased B Decreased Decreased Stay the same C Decreased Stays the same increased D Decreased Decreased decreased 9. In blood typing, the n-terminal sugar associated with A negative type blood is a) Galactose b) Glucosamine c) N-acetyl galactosamine d) N-acetyl glucosamine 10. If you were without a SPIROCRIT reader, you could still read a person’s HCT by: a) dividing the length of the whole blood sample by the length of the RBC portion b) dividing the length of the packed cell portion by the length of plasma sample in the tube. c) dividing the length of the packed cell portion by the length of whole blood sample in the tube. d) dividing the length of the buffy coat portion by the length of the blood sample in the tube. 11. In the following question, code in all letters that are correct. An “A positive” person is tested for blood type. We should see agglutination when we add the following antibodies to the drops of blood: a) anti -A b) anti –B c) anti- D (rh) 12. Rh HDN is a disease that is treated by transfusing __1__ blood into the _2___. 1 2 A Rh positive mother B Rh positive newborn C Rh negative mother D Rh negative newborn

13. The situation in which Rh HDN is a potential problem is when the pregnant mother is Rh __1_ and the fetus is Rh___2__. This results in the __3__ immune system going on the attack. 1 2 3 A positive negative baby’s B positive negative mother’s C negative positive baby’s D negative positive mother’s 14. RhoGam consists of __1__ and is given to the mother for the sake of neutralizing __2___. 1 st response 2 nd response A Rh antigen Fetal antigen B Rh antigen Fetal antibodies C Rh antibody Fetal antigen D Rh antibody Fetal antibodies 15. When an infant does have HDN, it is because __1___ crossed the placenta and caused severe ____2____. 1 st response 2 nd response A IgA’s Hemolysis B IgA’s Homeostasis C IgG’s Hemolysis D IgG’s Homeostasis 16. Two people arrive to donate blood. One is “A positive” and the other is “A negative.” Which of the following is true? A. The “A positive blood” can be donated to recipients who are “A positive” or “A negative” B. The “A negative blood” can be donated to recipients who are “A positive” or “A negative” 17. Bob has blood type A and his father has blood type O. Bob marries a woman with blood type AB. What are the possible blood types of their children? a) A and O b) A and B c) AB only d) B and O e) A, B, AB or O ab) A, B, or O ac) A, B, or AB 18. Martha wants to know if her next baby is at risk of having Rh HDN (erythroblastosis fetalis). She does not know her blood type, but she knows that her husband is Rh+ and that both of her parents were Rh-. Finally, she knows that her first child is Rh+. What would you tell her? a) She does not have to worry about HDN b) There is a 100% chance that her second baby will have a problem c) Her second child is at risk of having HDN; she should discuss this with her doctor d) Vaccines for HDN have eradicated this disease from continental Unites States 19. You isolated a patient’s agglutinins (antibodies) The patient has anti-A and anti-B agglutinins, but no anti-D agglutinins. The patient must be: a) A + b) A - c) B + d) B - e) O + ab) O - ac) AB + ad) AB – 20. Continuing with the above patient, this person can donate their blood to the following blood types:

A.) High, high B.) High, low C.) Low, high D.) Low, low 28. After testing we find that a patient’s HB is 14 gm%. How much oxygen can possibly be transported by this patient’s blood when it is 100% saturated with oxygen? A. 17.42 gm Oxygen/gm HB B. 17.42 ml Oxygen% C. 18.76 ml Oxygen % D. 18.76 gm oxygen/gm HB 29. What is true regarding agglutination versus coagulation? A. Agglutination involves platelets B. Coagulation requires clotting factors that come from red blood cells C. Agglutination is an immune response between an antigen and an antibody D. Coagulation involves negative feedback whereas agglutination involves positive feedback mechanisms. 30. Two expectant mothers sit at an OB/GYN office. The first mother has A negative blood and the father of her child has A positive blood. The second mother has blood type A positive blood and the father of her child has B negative blood. Which of the following is true? A. The fetus of the first mother is at risk of having HDN. B. The fetus of the second mother is at risk of having HDN. 31. You are a military medic, and a wounded soldier needs a blood transfusion. You have no way of blood typing the wounded soldier nor the other soldiers who are willing to donate their blood. On a piece of metal you mix a drop of blood from the wounded soldier with a drop of blood from a possible donor. You see agglutination occur. You conclude which of the following: A. The possible donor’s blood can be used to transfuse the wounded soldier; they are compatible blood types B. The possible donor should not donate blood to the wounded soldier; they are incompatible blood types Short Answer Responses: You have a patient with a Hb gm % of 19. This person also has a RBC of 7.5 x 106. The RBCs are normocytic. A. Calculate the patient’s oxygen carrying capacity with proper units (2 points). B. Discuss a possible reason for the patient’s condition and explain why you make this conclusion (2pts). Word Spell- Matching. Use the following choices to match to the descriptions below. Choices may be use more than once or not at all. Mark your answers below A. secondary polycythemia B. Hayem’s/Gower’s C. anemia D. Poikilocytosis E. Lymphocytes G. T3/ triiodothyronine L. T4/thyroxine M. Turk's N. humoral O. basophils P. eosinophils R. O- S. anisocytosis T. AB+ U. eosinophils Y. chromocytosis

36. _____ The active form of thyroid hormone 37. ______A pregnant woman would have elevated EPO levels and present with this condition 38. _______ The inactive form of Thyroid hormone 39. _______If you move to Denver, you may present with this condition. 40. _______ this condition is a reduced oxygen carrying capacity 41. _____ Universal recipient 42. ______ these cells mature into mast cells and begin inflammatory processes 43. _____ a microcytic cell would be an example of this term 44. _______ These cells mature into T cells and B cells