Biology 2290 Plasmid Mapping (1)

pdf

School

Western University *

*We aren’t endorsed by this school

Course

2290

Subject

Biology

Date

Feb 20, 2024

Type

pdf

Pages

Uploaded by JusticeNeutronAnt483

Biology 2290G Methods-Molecular Biology and Instrumentation Unit Plasmid Mapping to Determine Orientation of INSR Insert Digest Mackenzie Beddard

Purpose (/2) To determine the INSR inserts orientation using an agarose gel in addition to performing a digest on plasmid pAMP with enzymes EcoR1 and BamHI. Results (/5) Gel results for lane 1 are approximately a size of 11 KB in reference to the top band. For the middle band and bottom band, they are approximately 10 KB and 8 KB, respectively. For lane 2, the band is a size of approximately 6 KB, as well as lane 3, where the band as well is a size of 6 KB. The top band is 5 KB and the bottom band is about 1 KB.

Figure 1. Plasmid Mapping Results of Uncut, EcoR1, BamHI (lane 3), and double digest (EcoR1 and BamHI, lane 4). Discussion (/2) When analyzing the electrophoresis gel, orientation B of the double digest reflects a 3’-5’ direction where the INSR is being ligated into the plasmid. Lane 1 yielded 3 bands: one nicked (top), one linear (middle), and one supercoiled (bottom). The supercoiled is the smallest band, meaning it will migrate the easiest through the gel. Both lanes 2 and 3 were analyzed and evidently, both formed a single band. The plasmid size/insert were concluded to be 6 KB of single digests. In conclusion, the two lane single digest, and the uncut DNA nicked, linear, and

Your preview ends here

Eager to read complete document? Join bartleby learn and gain access to the full version

Access to all documents
Unlimited textbook solutions
24/7 expert homework help

supercoiled were evident. For the double digest, the insertion of the INSR into pAMP occurred in the wrong direction (3’-5’).

Related Documents

BIS2C Study Quiz #3 Microbial Eukaryotes.docx

Extensions of Basic Principles Group Problems (2).docx

Lab 3 - E- TLN Osmosis Part 1 (1).pdf

Basic Principles Group Problems (1).docx

Worksheet - CGS - Results of Crosses - updated FS2022.docx

BIO 154 Assignment 1, Chapters 1 and 2.docx

Week 3 Learning Activity.pdf

Microbiology Lab Unknown Project.pdf

Exam 1 Classroom Activities.pdf

Case Study 2 Lower GI.docx

Qualitative Tests for Biological Molecules Exercise 3 Lab.docx

Problem Set 3.docx

Recommended textbooks for you

Biochemistry

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Cengage Learning

Biology: The Unity and Diversity of Life (MindTap...

Biology

ISBN:9781305073951

Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Publisher:Cengage Learning

Human Heredity: Principles and Issues (MindTap Co...

Biology

ISBN:9781305251052

Author:Michael Cummings

Publisher:Cengage Learning

Biology: The Dynamic Science (MindTap Course List)

Biology

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Cengage Learning

Biology (MindTap Course List)

Biology

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Cengage Learning

Biochemistry

ISBN:9781305961135

Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:Cengage Learning

SEE MORE TEXTBOOKS

Recommended textbooks for you

Biochemistry
Biochemistry
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Cengage Learning
Biology: The Unity and Diversity of Life (MindTap...
Biology
ISBN:9781305073951
Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Publisher:Cengage Learning
Human Heredity: Principles and Issues (MindTap Co...
Biology
ISBN:9781305251052
Author:Michael Cummings
Publisher:Cengage Learning
Biology: The Dynamic Science (MindTap Course List)
Biology
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Cengage Learning
Biology (MindTap Course List)
Biology
ISBN:9781337392938
Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher:Cengage Learning
Biochemistry
Biochemistry
ISBN:9781305961135
Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:Cengage Learning