BIOCHEM LAB_At-Home 01 Lab Safety and Scientific Method

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Cincinnati State Technical and Community College *

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Biology

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Feb 20, 2024

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Integrated General Biology Name Laboratory At Home Instructor BIOCHEMISTRY At-Home Lab: Lab Safety and the Scientific Method Part A — Lab Safety The “at home” scientific laboratory should be a place of exploration and learning, and we must take every effort to minimize potential risks. Your safety in lab is the most important part of lab, even more important than what you are learning. You are expected to follow lab safety techniques while performing all activities. These include:  Follow all instructions carefully. If you do not understand a direction, ask for clarification.  Perform only the experiments authorized by the instructor.  Do not eat or drink during lab (even if it’s your home). Never use lab equipment as containers for food or beverages.  Always work in a well-ventilated area.  Dispose of all waste properly, as instructed.  Never mix household chemicals.  Do not touch your face, eyes, or mouth while working with chemicals.  Monitor your experiment at all times. Never leave experiments unattended.  Know the locations and how to operate lab safety equipment, such as fire extinguishers and eye wash.  Anytime chemicals, heat or glassware are used, eye glasses must be worn. Do not wear contact lenses in the laboratory.  Long hair must be tied back, and clothing must not be loose since these are hazards in the lab.  Shoes that cover the entire top of the foot must be worn.  Never taste or smell any chemicals.  Whenever working with a chemical, read the SAFETY DATA SHEET ( SDS ) before the experiment. Please complete the box below and electronically sign your name. I have read and I understand the lab safety rules. I agree to follow these rules and will ask questions before completing the activities if I do not understand any of the directions. Arriyon Simpson 02/09/2024 Arriyon Simpson PRINTED STUDENT NAME DATE STUDENT SIGNATURE © 2020 Socratis, LLC. This material may not be reproduced, displayed, modified or distributed without express prior permission. BIOCHEMISTRY At-Home Lab: Lab Safety and the Scientific Method 1

Integrated General Biology Name Laboratory At Home Instructor 1. Take a moment to describe your home-lab environment. What room is it? Is there open space? Will there be other family members that will be present? Please include notes about some important areas of concern or question, and let your instructor know about any possible problems. The are I will be using is located in my kitchen. I have 2 windows to open that will allow me to ventilate the area if needed. No other family memebers will be present because I live by myself. There is a lot of space in my kitchen in case I need to move around my dinning table. I do not have anything on my floor that will cause me to fall. 2. Draw and label your lab room (you won’t be graded on your artistic ability). Include any important areas and equipment. (Draw out your room, take a picture and insert it here.) 3. Safety Data Sheets (SDS) are often still referred to as Material Safety Data Sheets. Whatever you call them, they are a critical part of any safe lab. They provide necessary information on how to properly store, use and dispose of a chemical, and what to do in case of emergency. You will have to look up an SDS sheet for three different chemicals in lab this semester, on your own. One of these chemicals is iodine (or providone-iodine 10% solution). Since all SDS are set up the in the same order, any version should work. Find an SDS online for providone-iodine 10% solution. Review the entire document, then answer the questions below. What section is the first aid section (number)? What are the instructions for contact with the eyes? (Be sure to read the entire document to familiarize yourself with SDS.) Section 4: First aide measures:when in contact with eyes, immediately flush eyes with water for at least 15 minutes while holding eyelids open. If symptoms persist, get medical attention. © 2020 Socratis, LLC. This material may not be reproduced, displayed, modified or distributed without express prior permission. BIOCHEMISTRY At-Home Lab: Lab Safety and the Scientific Method 2

Integrated General Biology Name Laboratory At Home Instructor © 2020 Socratis, LLC. This material may not be reproduced, displayed, modified or distributed without express prior permission. BIOCHEMISTRY At-Home Lab: Lab Safety and the Scientific Method 3

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Integrated General Biology Name Laboratory At Home Instructor Part B — The Scientific Method Scientists, health care professionals, and the like, need to know a significant amount of background information to do their jobs. However, in most cases, critical thinking and reasoning skills are just as important as any background information. Being able to look at evidence and make logical conclusions that are based on that evidence is an invaluable tool in this kind of work, and in life. 1. You have been placed into lab groups. Each member of your team has been given two sides to a mystery cube. You will share that information with the other students later. For now, concentrate on your cube and the information on your two sides. Notice that the bottom is labeled. No one has information on the bottom side. Notes/Observations on your sides: The top of my cube has a 14 in the top left corner and a 4 in the bottom right corner, with 2 squares and 2 traingles. The other side of my cube has a 10 in the left top corner and a 3 in the bottom right corner with a square and 2 traingles in the middle. 2. Hold a brainstorming session with your team. Come up with a list of questions (at least 3) that your group has about the cube. Write them in the space provided here. Possible questions: 1. What does the other lab group members cube look like? 2. What is on the bottom of the cube? 3.Is there a pattern? © 2020 Socratis, LLC. This material may not be reproduced, displayed, modified or distributed without express prior permission. BIOCHEMISTRY At-Home Lab: Lab Safety and the Scientific Method 4

Integrated General Biology Name Laboratory At Home Instructor 3. Review your list of questions and STRIKETHROUGH (it’s the “abc “ button) any questions that are not testable, or ones that you cannot gather data on. For example, WHY questions are not readily testable, but it is possible to gather data on HOW, WHO, WHAT, WHEN, and WHERE. 4. While you may have other questions, the one we need to focus on is WHAT’S ON THE BOTTOM? This will be your question for the rest of this lab. 5. Go back and review your notes (evidence) that you collected with this question in mind. Circle all of the evidence that is appropriate for the question, and put a strikethrough any of the evidence that is not appropriate. Try to draw a net of the cube on a separate sheet of paper. It will help if everyone draws it the same way, like below. top sides side bottom 6. Review your observations. Circle any appropriate evidence that will help you answer the question and strikethrough any evidence that will not help you answer the question. 7. Complete the chart below to organize your thoughts and to help answer your question. Write out an important piece of evidence and explain what it means. Have at least 4 pieces of evidence with their corresponding claims. (Remember, a single claim may only answer the question partially and you will likely need several pieces of evidence and their explanations to give a complete answer.) Evidence (Observations) “I notice…” Claim (Explanation) “It means…” That there is a pattern with the shapes and numbers. We seen that there was two of each pattern and that the missin one could be the other missing pattern. © 2020 Socratis, LLC. This material may not be reproduced, displayed, modified or distributed without express prior permission. BIOCHEMISTRY At-Home Lab: Lab Safety and the Scientific Method 5

Integrated General Biology Name Laboratory At Home Instructor 8. Complete a net of the cube with the proper orientation (shown previously). Include what your group believes to be the answer to your question. Question: What is at the bottom of the cube? Answer: 14 at the top corner and 4 at the bottom corner with 2 squares and 2 traingles. Evidence (Patterns): We noticed that there was 2 of each patterns and there was a missing pattern partner. Analysis Hold a group discussion about the following questions and record your answers here. 1. How is your experience with the cubes similar to what scientists do? We formed a hypothesis that will help us discover what the meaning of each side of the cube could be,also come up with evidence and a claim. 2. What actions did you perform that are critical in the scientific method? We drew it out and made observations. © 2020 Socratis, LLC. This material may not be reproduced, displayed, modified or distributed without express prior permission. BIOCHEMISTRY At-Home Lab: Lab Safety and the Scientific Method 6

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Integrated General Biology Name Laboratory At Home Instructor 3. Is all evidence appropriate? Give an example of something that is evidence, but it not appropriate. Yes all of our evidence was appropiate to back up our claim. 4. Did you prove what is on the bottom of the cube? Can you be certain about what is on the bottom of the cube? Explain. Yes we did prove what was on the bottom of the cube because their was a pattern to each side and we figured out which pattern was missing. Closing Remarks Scientists ask questions (testable questions) about the world, and gather data from observations and experiments. Generally speaking, the more data one can collect, the stronger the proposed explanation will be. Scientists look for patterns in the data, and try to make predictions from these possible patterns. Then, they make their data public and invite feedback from other scientists. The other scientists will critique the work and try similar experiments to see if they agree with the findings. All of this data collected either supports the proposed explanation, or it refutes it. Science actually never proves anything; it simply gathers data and then determines if that data supports a hypothesis or not. You are starting a journey into the world of science. Scientists make mistakes all the time, we all do. Try not to be overly concerned about any mistakes you may make, but rather focus in on learning from your experiences. This lab is designed to be a community of learners and you should feel comfortable sharing your thoughts here. Enjoy your new journey. © 2020 Socratis, LLC. This material may not be reproduced, displayed, modified or distributed without express prior permission. BIOCHEMISTRY At-Home Lab: Lab Safety and the Scientific Method 7

BIOCHEM LAB_At-Home 01 Lab Safety and Scientific Method

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