AA3 Biochemical assignment

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South Dakota State University *

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Biology

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Feb 20, 2024

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Uploaded by ProfWasp4094

Micro231L: Spring 2023 Activity Assessment 3: Worth 5 pts Biochemical and Selective/Differential Media Assignment The assignment is due in D2L Dropbox 12/4/23 by 11:59 pm 1. Obligate anaerobes are often grown in an anaerobe jar, which completely excludes oxygen from the environment. How is the environment within a tube of fluid thioglycollate media different from that found within the anaerobe jar? 2. Differentiate between the terms selective and differential as they apply to mannitol salt agar. 3. What would be the effect of removing the sodium chloride from mannitol salt agar plates? 4. Differentiate between the terms selective and differential as they apply to MacConkey agar. 5. Differentiate between the terms selective and differential as they apply to eosin methylene blue 6. Differentiate between the terms selective and differential as they apply to xylose lysine deoxycholate. 7. How could MacConkey agar aid in the identification of coliforms? (definition of coliform is in the lab manual). 8. Phenol red is another indicator commonly used to detect the presence of acid. This indicator is red above pH7 and yellow below pH of 7. What would be the appearance of E. coli (gram-negative rod, lactose fermenter) and S. aureus (gram-positive cocci, lactose fermenter) if neutral red in MacConkey agar was replaced with phenol red?

9. What is sheep blood agar classified as enriched, selective, differential, general-purpose? (could be more than 1) Explain the reasoning behind the terms picked. 10. Why was important to inoculate motility medium with a single stab line? 11. Motility medium consist of 0.4% agar, what would you expect to happen if the medium was made with 2% agar instead? 12. TCC is a chemical that is added to a motility medium to make the motility of bacteria easier to see because it turns red when it is oxidized. Imagine your lab partner comes to you and complains that they cannot see the stab line because the entire tube is obscured by the red color of the TCC. What would you tell them? 13. What are the metabolic products being tested for in the methyl-red and Voges-Proskauer test? 14. Methyl-red test and Voges-Proskauer test are made up of the same media formulation (the same). How is it possible to detect two different reactions using the same media? 15. Hydrogen peroxide is used as an antiseptic to kill bacteria in cuts and abrasions. Many of these bacteria are catalase-positive, yet they are still killed by the actions of hydrogen peroxide. Why does this occur?

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