Lab 22 - Nematode Experiment Log

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Georgia State University *

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1104L

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Biology

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Feb 20, 2024

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Name: Hafsah, Sara, Raelee, Lab 22 – Nematode Experiment Log This experiment is done over two days. You will need to submit your complete worksheet on iCollege by November 21 st at 10:00 a.m. Day 1: Wet weight of reference sample in grams: Before soil: 2.23, 2.19, 2.19 After soil: reference: 20.56 (before) – 2.23 = 18.33 Nematode (funnel) : 22.26, 25.67 Hypothesis (How will the Soil Density affect the number of nematodes in a sample? How about water percentage? Organic Material? Make three hypotheses): Hypothesis #1: The denser the soil is the more nematodes there will be. Hypothesis #2: The higher the water percentage is the more nematodes there will be in the soil. Hypothesis #3: There won’t be a lot of nematodes in the soil because organic material is limited since we only have the soil sample. Independent variable: Soil density, water percentage, humus Dependent variable: How many nematodes are found. Control variables: Height of the soil (3 cm), water (funnel) Day 2: Soil Density (Dry weight of reference sample):

Organic Matter (Humus Absorbance at 540 nm): Average number of nematodes: Water Percentage = (1 – Dry weight/wet weight) X 100% : Class Data: Average Number of Nematodes Organic Matter (540 nm) Soil Density (g) Water Percentage (%) Group 1 Group 2 Group 3 Group 4 1. Place all data in Excel by group and convert each column of raw data into “ranked data” where each group is ranked in order with 1 being the least. 2. Create three XY scatter plot graphs: Soil Density vs. Number of Nematodes, Water Percentage vs. Number of Nematodes, and Organic Matter vs. Number of Nematodes. 3. Display a linear trend line and R 2 value for each graph. Here is a website for a quick review on how to display both on your graphs: https://www.ablebits.com/office-addins- blog/2019/01/09/add-trendline-excel/ Graph #1: Soil Density vs. Number of Nematodes Positive or negative correlation? Is the trend line a “good fit”? Interpret R 2 . Graph #2: Water Percentage vs. Number of Nematodes Positive or negative correlation? Is the trend line a “good fit”? Interpret R 2 . Graph #3: Organic Matter vs. Number of Nematodes Positive or negative correlation? Is the trend line a “good fit”? Interpret R 2 .

Conclusions: What correlations did you observe between nematode number and the three soil features you measured? Were your hypotheses correct?

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