Noyes_C_M4Worksheet

M4 WORKSHEET NEURAL PHYSIOLOGY Name: __Casey Noyes__________ Section: __3_________________ Station: __1_________________ Date: _2/1________________ E1: NEURON FIBER RECRUITMENT 1. Identify the following or write NA if not applicable to E1. (2 pts each) a) Independent Variable = _stimulus voltage___________________ b) Dependent Variable = __CAP amplitude__________________ a) Controlled Variable = ____________________ b) Threshold Stimulus Voltage = _160______________ mV c) Maximal Stimulus Voltage = __270_____________ mV 2. Complete Table 1 and identify the following using data acquired during E1. (20 pts) Table 1: Exercise 1 results. Stimulus Voltage (mV) CAP Amplitude (mV) 10 0 20 0 30 0 40 0 50 0 60 0 70 0 80 0 90 0 100 0 110 0 120 0 130 0 140 0 150 0 160 0.684 170 1.206 180 2.388 190 4.102 200 7.220 210 8.878 220 10.347 1 Boswell LC (Spring 2024) Vertebrate Physiology Student Lab Manual . Baton Rouge, LA: Louisiana State University. This material is provided for the educational benefit of students currently enrolled in BIOL 4161 at LSU and is restricted from further use. Portions of material are copied and/or adapted from ADInstruments Student Protocols (Copyright © ADInstruments Pty Ltd). Published materials, such as textbook images, are included for nonprofit educational purposes only under the fair use exemption and are restricted from further use. The posting of course material to any form of note-sharing website or online database is strictly prohibited.

M4 WORKSHEET 230 11.642 240 12.239 250 12.702 260 13.028 270 13.397 280 13.569 290 13.736 300 13.847 E2: TEMPORAL SUMMATION 3. Identify the following or write NA if not applicable to E2. (2 pts each) a) Independent Variable = _ISI___________________ b) Dependent Variable = __CAP amplitude__________________ c) Controlled Variable = __stimulus voltage__________________ d) Threshold Stimulus Voltage = _160______________ mV e) Sub-threshold Stimulus Voltage = _140_______________ mV 4. Complete Table 2 and identify the following using data acquired during E2. (20 pts) Table 2: Exercise 2 results. ISI (  s) CAP Amplitude (mV) 1000 0 900 0 800 0 700 0 600 0 500 0.153 400 0.561 300 1.270 200 2.436 100 4.423 90 4.544 80 4.495 70 7.313 60 6.961 50 7.352 40 7.281 30 7.459 20 11.481 10 11.461 2 Boswell LC (Spring 2024) Vertebrate Physiology Student Lab Manual . Baton Rouge, LA: Louisiana State University. This material is provided for the educational benefit of students currently enrolled in BIOL 4161 at LSU and is restricted from further use. Portions of material are copied and/or adapted from ADInstruments Student Protocols (Copyright © ADInstruments Pty Ltd). Published materials, such as textbook images, are included for nonprofit educational purposes only under the fair use exemption and are restricted from further use. The posting of course material to any form of note-sharing website or online database is strictly prohibited.

M4 WORKSHEET E4: CONDUCTION VELOCITY 7. Record the measured distance between recording electrode1 Channel 1 and recording electrode 1 Channel 2 = ___5____________ mm (4 pts) 8. Complete Table 4 using data acquired during E4. (10 pts) Table 4: Exercise 4 results. Replicate Time required for CAP to travel the measured distance (ms) Conduction Velocity (m/s) 1 0.49 10.204 2 0.48 10.417 3 0.49 10.204 4 Boswell LC (Spring 2024) Vertebrate Physiology Student Lab Manual . Baton Rouge, LA: Louisiana State University. This material is provided for the educational benefit of students currently enrolled in BIOL 4161 at LSU and is restricted from further use. Portions of material are copied and/or adapted from ADInstruments Student Protocols (Copyright © ADInstruments Pty Ltd). Published materials, such as textbook images, are included for nonprofit educational purposes only under the fair use exemption and are restricted from further use. The posting of course material to any form of note-sharing website or online database is strictly prohibited.