Class 21 FRAP Worksheet

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University of Utah *

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1610-001

Subject

Biology

Date

Feb 20, 2024

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pdf

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1

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Student name and uNID_____________________________________________________________________ Biol 1610 Class 21 FRAP analysis worksheet The graph below shows the results of a FRAP analyses studying M embrane P rotein 1-4 (MP1-MP4). Work with your group to answer the questions to the right of each graph. 1. Label the graph with an arrow to show when the laser bleached the fluorescent protein ( MP1 ). 2. Did the MP1 protein move to restore its previous distribution? YES // NO (circle one) 3. For question 2, what was your reasoning for selecting your answer? 4. Did MP2 move to restore its previous distribution? YES // NO (circle one) 5. How does MP2's movement compare to that of MP1 ? faster // same // slower (circle one) Why do you think this is true? 6. Did MP3 move to restore its previous distribution? YES // NO (circle one) 7. For question 6, what was your reasoning for selecting your answer? 8. Did MP4 move to restore its previous distribution? YES // NO (circle one) 9. Use your knowledge of membrane proteins to propose a model that explains why proteins would show the FRAP pattern of MP4
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