Homework Assignment for Lab 9 (Part 2) (1)

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May 28, 2024

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Appolon 1 Odethny Appolon BIOL 1500 Lab – E02 9 April 2024 Homework Assignment for Lab 9 (Part II) 1. Why does DNA move through an agarose gel? a. DNA moves through an agarose gel because it has a negative charge. The electric current will push the DNA towards the positive electrode once it is in the gel. 2. What is an allele ladder? What is its function in DNA profiling? a. An allele ladder is a reference marker, and it allows you to compare the relative sizes and identities of DNA. 3. What is required to visualize DNA following electrophoresis? a. An orange loading dye, agarose gel, and a buffer. 4. Draw/insert an image of your gel. What was your test food? What was the GMO status of your sample? a. My test food was cereal. The GMO status was positive. 5. In the example shown below, the allele assignment for the sample in lane 2 is 5-2, since there is one allele 5 and one allele 2 in that lane. Write down the genotype for each of your samples in the following table. Lane Sample Number of Bands? Genotype (BXP007 Alleles Present) 1 BXP007 Allele Ladder 8 15,10,7,5,4,3,2,1 2 Crime Scene DNA 2 5-2 3 Suspect A DNA 2 7-4 4 Suspect B DNA 2 5-2 5 Suspect C DNA 2 7-2 6 Suspect D DNA 2 10-3 6. Does the Crime Scene DNA sample have a genotype that matches any of the suspects? If so, which one matches? a. Yes, the crime scene DNA sample’s genotype matches suspect B.

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