Evaluation of Antiseptics and Disinfectants Questions1

docx

School

Otero Junior College *

*We aren’t endorsed by this school

Course

204

Subject

Biology

Date

May 26, 2024

Type

docx

Pages

Uploaded by shanromer1714

1 Evaluation of Antiseptics and Disinfectants answer Sheet Pre-Laboratory Questions 1. What is the difference between an antiseptic and a disinfectant? Antiseptics are substances that are used to reduce microbe levels on or in living tissues Disinfectants are substances that are used to reduce microbe levels on inanimate, nonliving surfaces or in liquids. 2. Would a disinfectant necessarily kill bacterial endospores? Why or why not? A disinfectant wouldn’t necessarily kill bacterial endospores but would reduce the number of microbes. Only sterilization would kill bacterial endospores, this would include the use of high-pressured heat to kill them or harsh chemicals. 3. List five factors that can influence the effectiveness of antimicrobial agents. Five factors that can influence the effectiveness of antimicrobial agents include: 1. The type of microbe. Some microbes are more resilient than others. 2. Number of microbes. If the number of microbes is high there’s a greater chance that one or more may survive and repopulate. 3. The concentration of the antimicrobial chemical. 4. Temperature. 5. Nature of the surface or material to be treated. 4. Several antimicrobial agents denature proteins. What does this mean and how does protein denaturation affect microbes? Denaturing is the change to the structure of the protein. The protein denaturation affects the microbes killing normal protein function and this causing the death of microbes. ©2016 Carolina Biological Supply Company

2 Observations Data Table 1: Disinfectant and antiseptic concentrations and bacterial growth Fill out the table below based on the instructions listed in the lab Treatment Growth Control ++ 0.01% Bleach - 0.1% Bleach - 1.0% Bleach - 25% Lysol ® - 50% Lysol ® - 100% Lysol ® - 0.03% Hydrogen Peroxide ++ 0.3% Hydrogen Peroxide + 3.0% Hydrogen Peroxide - 10% Isopropyl Alcohol + 30% Isopropyl Alcohol - 50% Isopropyl Alcohol - ©2016 Carolina Biological Supply Company

3 Observations Take a picture of each tube at the designated time, and insert them below (be sure to label each photo with organism, name of disinfectant or antiseptic, incubation time and temperature : LYSON 48 HOURS ©2016 Carolina Biological Supply Company

Your preview ends here

Eager to read complete document? Join bartleby learn and gain access to the full version

Access to all documents
Unlimited textbook solutions
24/7 expert homework help

Your preview ends here

Eager to read complete document? Join bartleby learn and gain access to the full version

Access to all documents
Unlimited textbook solutions
24/7 expert homework help

7 ISOPROPYL ALCOHOL Post-lab Questions 1. Based on your results, which antimicrobial agent(s) best inhibited the growth of E. coli ? What was the lowest effective concentration of the agent(s)? ©2016 Carolina Biological Supply Company

8 The antimicrobial agents that inhibited the growth of E. coli the best was Lysol. The least effective agent was Hydrogen peroxide. 2. What can you conclude from your results about the relative effectiveness of antiseptics vs. disinfectants in preventing the growth of E. coli ? Based on my results I concluded that disinfectants are more effective against E. coli than antiseptics. 3. Why is it useful to know and use the lowest effective concentration of an antimicrobial chemical? It is useful to know and use the lowest effective concentration of an antimicrobial chemical in order to know which strength is effective at killing microbe. Using the lowest effective concentration allows the chemical to kill the microbes effectively yet has the potential to cause less harm. 4. Were there any discrepancies between what you saw in your experiment and what you expected to see based on the lab manual and your readings in this section? I expected the “control” tube to have the most growth, which did happen. I also expected the bleach tubes to have the least amount of growth which was what occurred. ©2016 Carolina Biological Supply Company

Related Documents

Phlebotomy review #1.docx

chapter 47 Review Questions.docx

chapter 44 Review Questions.docx

Lesson 19 GRQ.docx

Hand Washing Techniques Questions.docx

Tutorial 3_S24_AnswerKey.docx

Cell Bio Task 4.docx

Homework Assignment for Lab 9 (Part 2) (1).docx

BIO 121 Exam #1 (Spring 2024)- Versions A and B Key (1).docx

Lab C - Lab Manual Chapter_ 24W-LIFESCI-23L-LEC-1 Introduction to Laboratory and Scientific Methodol

LAB 1 - Working With Excel.pdf

Pre-Lab quiz 1-Spinal cord: Sp24 BIOL F040B Human Anatomy & Physiology Ii 03Y, 04Y Mousavi Shafaei 4

Recommended textbooks for you

Principles Of Pharmacology Med Assist

Biology

ISBN:9781337512442

Author:RICE

Publisher:Cengage

Biomedical Instrumentation Systems

Chemistry

ISBN:9781133478294

Author:Chatterjee

Publisher:Cengage

Microbiology for Surgical Technologists (MindTap ...

Biology

ISBN:9781111306663

Author:Margaret Rodriguez, Paul Price

Publisher:Cengage Learning

Basic Clinical Laboratory Techniques 6E

Biology

ISBN:9781133893943

Author:ESTRIDGE

Publisher:Cengage

Surgical Tech For Surgical Tech Pos Care

Health & Nutrition

ISBN:9781337648868

Author:Association

Publisher:Cengage

Complete Textbook Of Phlebotomy

Biology

ISBN:9781337464314

Author:Hoeltke

Publisher:Cengage

SEE MORE TEXTBOOKS

Recommended textbooks for you

Principles Of Pharmacology Med Assist
Biology
ISBN:9781337512442
Author:RICE
Publisher:Cengage
Biomedical Instrumentation Systems
Chemistry
ISBN:9781133478294
Author:Chatterjee
Publisher:Cengage
Microbiology for Surgical Technologists (MindTap ...
Biology
ISBN:9781111306663
Author:Margaret Rodriguez, Paul Price
Publisher:Cengage Learning
Basic Clinical Laboratory Techniques 6E
Biology
ISBN:9781133893943
Author:ESTRIDGE
Publisher:Cengage
Surgical Tech For Surgical Tech Pos Care
Health & Nutrition
ISBN:9781337648868
Author:Association
Publisher:Cengage
Complete Textbook Of Phlebotomy
Biology
ISBN:9781337464314
Author:Hoeltke
Publisher:Cengage