Monohybrid Cross BIOLOGY SIMULATION 2

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May 22, 2024

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www.biologysimulations.com Monohybrid Cross: Identifying Inheritance Pattern Background: You will be studying two traits in a fictional mammal-like organism. The wild type phenotype is black eyes and tailless. White eyes and tails are mutant traits. All P generation organisms are assumed to be homozygous. I. White Eyes Question: What is the inheritance pattern of the white eye mutation? 1. Select a P generation pair to test for the inheritance pattern of white eyes. You can choose from the shaded options indicated on the grid. Since we are testing for the inheritance of white eyes, the presence or absence of a tail is not important to this cross. 2. Wild Type Female x Wild Type Male Tail Female x Tail Male Tail Female x Wild Type Male Wild Type Female x Tail Male Wild Type Female x White Eyes Male Tail Female x White Eyes/Tail Male Tail Female x White White Eyes Male Wild Type Female x White Eyes/Tail Male White Eyes Female x Wild Type Male White Eyes/Tail Female x Tail Male White Eyes/Tail Female x Wild Type Male White Eyes Female x Tail Male White Eyes Female x White Eyes Male White Eyes/Tail Female x White Eyes/Tail Male White Eyes/Tail Female x White Eyes Male White Eyes Female x White Eyes/Tail Male P generation cross: _________________________ x _________________________ 3. Record the results. F1 Results: Count % Black Eye Females Black Eye Males White Eye Females White Eye Males 4. Select the F1 cross F1 generation cross: _________________________ x _________________________

www.biologysimulations.com 5. Make a chart to record the F2 results. F2 Results: Count % Black Eye Females Black Eye Males White Eye Females White Eye Males Analysis: 6. Is there a significant difference in expression of the white eye trait in males and females in either the F1 or F2 results? 7. What are the genotypes of the P generation cross? P generation cross: _______________ x _______________ 8. Based on the F1 generation results, which allele (white or black) is dominant? Use a Punnett square to demonstrate your reasoning. 9. What is the genotype of F1 individuals? 10. Use a Punnett square to show the F1 cross and determine the expected F2 phenotype distribution. F2 Expected:

www.biologysimulations.com Expected % Black Eye Females Black Eye Males White Eye Females White Eye Males 11. Compare your expected % to the observed F2 data. Is the data consistent with your hypothesis of the inheritance pattern (from question 9)? II. Tail Use the same process as in Part I to determine the inheritance pattern of the tail trait. P generation cross: _________________________ x _________________________ F1 Results: Count % Tail Females Tail Males Tailless Females Tailless Males F1 generation cross: _________________________ x _________________________ F2 Results:

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www.biologysimulations.com Count % Tail Females Tail Males Tailless Females Tailless Males Analysis: F2 Expected: Expected % Tail Females Tail Males Tailless Females Tailless Males What is the inheritance pattern for this trait? Explain the evidence for your conclusion. You may use Punnett squares as part of your explanation.

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