Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Question
Chapter B, Problem 2P
Summary Introduction
To determine:
The serial dilution in the given samples.
Introduction:
The stepwise dilution of a solution is termed as a serial dilution. Generally, the dilution factor at each stage of serial dilution is constant and leads to geometric progression in a logarithmic manner of the concentration of the sample. Serial dilutions are used to dilute the given substance at a very high level and serial dilutions are also used in the formation of concentration curves.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
Question: Determine the amount of dehydrated medium needed to prepare 15 butts, 20 slants, and 25 butt-slants. Include amount for 2 additional units of each as excess to compensate for compounding losses.
1. Calculate the MAWQ for an electronic balance with a linearity of 0.001 g and an
acceptable error of 5% or 0.05.
The analysis of urea in the urine sample is what type of analysis?
O Complete analysis
O Proximate analysis
O Ultimate analysis
O Ultramicro analysis
« Previous
Chapter B Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- FOLIN-WU METHOD a. What is the type of blood sample used and how much of it is added to distilled water? b. What are the protein precipitants used and indicate the specific concentration of each? c. What is the color of the precipitate obtained? d. If the desired color of the mixture(blood sample and reagent mixture) is not achieved, what chemical is added drop by drop to obtain the desired color? Specify the conc.arrow_forwardAnswer the following problem using Reducing and Enlarging Formula (Factor Method)arrow_forward2. Calculate the following: a. you are asked to prepare 10 NA slants and 5 NA stab in big tubes. How much NA should you prepare if the indicated preparation for NA is as follows: 1. Suspend 28g of nutrient agar powder (CM0003B) in 1L of distilled water. 2. Mix and dissolve them completely. 3. Sterilize by autoclaving at 121°C for 15 minutes.arrow_forward
- EXPERIMENT :DETERMINATION OF CHEMICAL OXYGEN DEMAND (COD) 1. OBJECTIVES To determine the chemical oxygen demand of the given wastewater samples. 2. PROCEDURE Preparing the Sample 1. Open a reaction vial with a white cap and add the specified volume. (Ensure that appropriate personnel safety equipment is used.) LR-/MR: 2 ml water sample HR: 0.2 ml water sample 2. Prepare a blank by using deionised water (TOC-free) instead of the sample (LR/MR: 2 ml, HR: 0.2 ml). 3. Replace the cap tightly. Invert the vial gently several times to mix the contents (The vial will become hot during mixing!) and digest the vials for 120 minutes in the reactor at a temperature of 150°C. 4. Remove the vials from the reactor and allow them to cool down to 60°C or less. Mix the contents by inverting each vial several times while still warm. Then allow the vials to cool to ambient temperature before measuring. 3:Results Sample COD reading (mg/L) Leachate 216 Factory 423…arrow_forwardDescription Tabulate the different preservatives used for urine specimen and indicate their advantages and disadvantages Tabulate the different types of urine specimen and the collection method and the tests when they are indicated A specimen with a pH of 9 was received in the laboratory. Should you accept it or not? If not, what should you do? Explain your answer. A urine specimen has a specific gravity of 1.032 obtained through refractometer. The temperature of the specimen is 25 degrees C with 1 g/dL of protein and 2 g/dL of glucose. What is the corrected specific gravity? Show your solution.arrow_forwardA 15kg doe diagnosed with pregnancy toxaemia requires a dextrose fluidsupplementation. Given a dosage of 0.2ml/kg of body weight, prepare a 5%dextrose solution from a stock solution of 50%.arrow_forward
- 1. Determine the serial dilution used from the original water sample (100 mL) with 4.8 x 105 CFU asshown in the diagram.arrow_forward750mL mL of a dextrose solution is to be infused at 45 mL/h starting at 1600 hours. At what time will this IV be completed?arrow_forward21. R Rhus toxicodendron extract 10 mg/ 100 mL Sterile water for injection q.s. Sig: as directed How many milliliters of a 100 mg/mL concentrate of Rhus toxicodendron extract should be used in preparing the prescription?arrow_forward
- Norcuron .05mg/kg is ordered IVP. The patient weighs 145lbs. Norcuron issupplied 1mg/5ml. How many cc’s will be administered IVP? The order reads: NS 1.5 liters over 12 hours. You choose gravity tubingwith a drip factor of 20gtts/cc. How many gtts/min will you set the fluids toinfuse at?arrow_forwardExperiment No. 6 Milk.pdf - Adobe Acrobat Reader DC File Edit View Sign Window Help Home Tools Experiment No. 6 .. x 玩, 2 14 116% 3. Formation and Composition of Film from Milk a. Place 10 mL of fresh milk in an evaporating dish. b. Boil and observe the formation of film. Observation: c. Remove the film and then boil again. Observation: d. Test the film with Biuret's test and Acrolein test. Search Signa Convert E Edit PDF Lo Create PDF Test Performed Result with Milk Film Substance present Biuret's test Adobe PDF Pack Acrolein test Convert files to PDF anc easily combine them wit other file types Guide Questions: 1. What can you conclude regarding the chemical composition of the film formed? Select File to Convert to P e. Get 10 mL fresh milk, then acidify it with acetic acid, and repeat the above experiment. Select Fite 2. Did you get a film on boiling sour milk? 3. Explain the mechanism involved in the formation of film. Create, edit and sign PDF forms & agreements Start Free Faf B.…arrow_forward2 if there is a stock solution of 2000 (ug/ml) , is it possible to use SERIAL DILUTION to generate the following 8 tubes with their concentrations? A 2000 (ug/ml) B 1500 (ug/ml) C 1000 (ug/ml) D 750 (ug/ml) E 500 (ug/ml) F 250 (ug/ml) G 125 (ug/ml) H 0 (ug/ml)arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
