Inquiry into Life
Inquiry into Life
14th Edition
ISBN: 9780073525525
Author: Mader, Sylvia S./
Publisher: McGraw-Hill College
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Chapter 9.6, Problem 1LO

Identify the structures of a leaf.

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You intend to insert patched dominant negative DNA into the left half of the neural tube of a chick.  1) Which side of the neural tube would you put the positive electrode to ensure that the DNA ends up on the left side?  2) What would be the internal (within the embryo) control for this experiment?  3) How can you be sure that the electroporation method itself is not impacting the embryo?  4) What would you do to ensure that the electroporation is working? How can you tell?
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General Embryology Review in 20 minutes; Author: Medical Animations;https://www.youtube.com/watch?v=4YKvVeVMmEE;License: Standard youtube license