EBK LEHNINGER PRINCIPLES OF BIOCHEMISTR
7th Edition
ISBN: 8220103662253
Author: nelson
Publisher: MAC HIGHER
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Chapter 9, Problem 9P
Summary Introduction
To determine: The reason behind using two antibodies successively for detection of a protein and the reason why one is labeled and why not using only one antibody.
Concept introduction:
Immunofluorescence is a microscopic technique used for identification of proteins by exploiting its antigenic property. This property is based on protein-protein interaction. The compound used for observation is a fluorophore bounded to an antibody which is specific to the antigen.
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In the Thermo Fisher application note about wine analysis (Lesson 3), the following
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MAU
300
200
T
34
5
100-
1 2
CO
6
7
8
9
0
2.4
2.6
2.8
3.0 3.2 3.4
3.6
3.8 4.0 4.2
4.4
4.6
4.8
5.0
5.2
Minutes
3.22
0.62
1.04
O 1.24
The diagram shows two
metals, A and B, which melt at
1000°C and 1400°C. State the
weight percentage of the
primary constituent (grains of
C) that would be obtained by
solidifying a 20% alloy of B.
1000°C
a+L
L+C
900°С
12
α
a+C
45
1200 C
L+y
140096
C+Y
a+ß
800°C
700°C
C+B
96
92
a+B
0
10
20
30
40
50
60
70 80 90 100
A
% peso B
B
8.
Choose the compound that will produce the spectrum below and assign the signals to the corresponding
protons.
2
4
3
ō (ppm)
OH
4
6 6
СОН
2
1
0
Chapter 9 Solutions
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Immune System and Immune Response Animation; Author: Medical Sciences Animations;https://www.youtube.com/watch?v=JDdbUBXPKc4;License: Standard YouTube License, CC-BY
Immune response: summary; Author: Dr Bhavsar Biology;https://www.youtube.com/watch?v=ADANgHkX4OY;License: Standard Youtube License