
EBK LEHNINGER PRINCIPLES OF BIOCHEMISTR
7th Edition
ISBN: 8220103662253
Author: nelson
Publisher: MAC HIGHER
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Chapter 9, Problem 9P
Summary Introduction
To determine: The reason behind using two antibodies successively for detection of a protein and the reason why one is labeled and why not using only one antibody.
Concept introduction:
Immunofluorescence is a microscopic technique used for identification of proteins by exploiting its antigenic property. This property is based on protein-protein interaction. The compound used for observation is a fluorophore bounded to an antibody which is specific to the antigen.
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EBK LEHNINGER PRINCIPLES OF BIOCHEMISTR
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