EBK LEHNINGER PRINCIPLES OF BIOCHEMISTR
7th Edition
ISBN: 8220103662253
Author: nelson
Publisher: MAC HIGHER
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Chapter 9, Problem 2P
Summary Introduction
To determine: How to identify recombinant yeast artificial chromosome (YAC) from other plasmid.
Introduction:
A vector is a DNA molecule which is used as a vehicle to transport foreign genetic material into the target cell. In target cell, it can be replicated and get expressed. A vector that contains a foreign DNA is termed as a recombinant DNA. The four classes of vectors are plasmids, cosmids, viral vectors, and artificial chromosomes.
DNA cloning is a technique which is used to make genetically identical copies of a gene or segment of DNA. Recombinant DNA technology allows the cloning of DNA to make recombinants. Vector such as plasmid used for cloning purposes. The desired gene can be inserted into the bacterium (host cell).
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In the Thermo Fisher application note about wine analysis (Lesson 3), the following
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MAU
300
200
T
34
5
100-
1 2
CO
6
7
8
9
0
2.4
2.6
2.8
3.0 3.2 3.4
3.6
3.8 4.0 4.2
4.4
4.6
4.8
5.0
5.2
Minutes
3.22
0.62
1.04
O 1.24
The diagram shows two
metals, A and B, which melt at
1000°C and 1400°C. State the
weight percentage of the
primary constituent (grains of
C) that would be obtained by
solidifying a 20% alloy of B.
1000°C
a+L
L+C
900°С
12
α
a+C
45
1200 C
L+y
140096
C+Y
a+ß
800°C
700°C
C+B
96
92
a+B
0
10
20
30
40
50
60
70 80 90 100
A
% peso B
B
8.
Choose the compound that will produce the spectrum below and assign the signals to the corresponding
protons.
2
4
3
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OH
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6 6
СОН
2
1
0
Chapter 9 Solutions
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