Microbiology: An Introduction
11th Edition
ISBN: 9780321733603
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: Benjamin Cummings
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Textbook Question
Chapter 9, Problem 9MCQ
Self-replicating DNA for transmitting a gene from one organism to another.
- a. antisense
- b. clone
- c. library
- d. Southern blot
- e. vector
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Match items in Column A with most related ones in B. Write the letters on the space provided.
Some strains of Escherichia coli bacteria have acquired the ability to produce the harmful Shiga toxin, normally produced by Shigella dysenteriae. Which statements best explain why this occurs? Mark all that apply.
A. conjugation between two different species of bacteria allowed both strains of bacteria to express a virulence factor
B. transformation resulted in expression of a virulence factor
C. horizontal gene transfer between different species of bacteria
D. transduction of a specific gene resulted in expression of new traits
In the 1953 study by Hershey and Chase
A. 32P was found in supernatants after T2 infection
B. 35S was found in bacterial pellets after T2 infection
C. E. coli genomes were inherited after T2 infection
D. RNA was excluded as THE heritable material
E. T2 coats were removed with a blender after initial phage binding
Chapter 9 Solutions
Microbiology: An Introduction
Ch. 9 - Compare and contrast the following terms: a. cDNA...Ch. 9 - Differentiate the following terms. Which one is...Ch. 9 - Some commonly used restriction enzymes are listed...Ch. 9 - Suppose you want multiple copies of a gene you...Ch. 9 - Which enzyme makes the smallest fragment...Ch. 9 - Describe a recombinant DNA experiment in two or...Ch. 9 - List at least two examples of the use of rDNA in...Ch. 9 - You are attempting to insert a gene for saltwater...Ch. 9 - How does RNAi silence a gene?Ch. 9 - Prob. 10R
Ch. 9 - Restriction enzymes were first discovered with the...Ch. 9 - The DNA probe, 3-GGCTTA, will hybridize with which...Ch. 9 - Which of the following is the fourth basic step to...Ch. 9 - The following enzymes are used to make cDNA. What...Ch. 9 - If you put a gene in a virus, the next step in...Ch. 9 - You have a small gene that you want replicated by...Ch. 9 - Pieces of human DNA stored in yeast cells. a....Ch. 9 - A population of cells carrying a desired plasmid....Ch. 9 - Self-replicating DNA for transmitting a gene from...Ch. 9 - A gene that hybridizes with mRNA. a. antisense b....Ch. 9 - Design an experiment using vaccinia virus to make...Ch. 9 - Why did the use of DNA polymerase from the...Ch. 9 - The following picture shows bacterial colonies...Ch. 9 - Prob. 1CAECh. 9 - Using the restriction enzyme ECORI, the following...
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- Why are vaccines and/or passive immunization the method of choice in the treatment or prevention of viral infections? A. There are no known inhibitors to reverse transcription B. Viruses are non-living and do not have their own genetic mechanisms to reproduce by themselves hence host cells must rely on targeting the virus or their products. C. Antibodies prevent replication of viral genome D. Antibodies prevent transcription and translation of viral genomes E. All of thesearrow_forwardMatch the following terms on the left with those on the right. Not all will be used and some may be used more than one time. priononcogenemRNAviroids a. -sense RNAb. housekeeping genec. Infectious protein d. Infectious RNAe. cancer causingf. + sense RNAarrow_forwardWhen you performed the phage titer experiment you performed several dilutions of the original stock. Which plate was useful? a. the dilution that produces the plate with no visible virus b. the plate with fewer than 25 viral infections of E. coli c. the plate with the most dense virus d. the plate with between 30 and 300 visible viral infections of E. coliarrow_forward
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