Microbiology: An Introduction
11th Edition
ISBN: 9780321733603
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: Benjamin Cummings
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Chapter 9, Problem 2MCQ
The DNA probe, 3′-GGCTTA, will hybridize with which of the following?
- a. 5′-CCGUUA
- b. 5′-CCGAAT
- c. 5′-GGCTTA
- d. 3′-CCGAAT
- e. 3′-GGCAAU
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The restriction enzyme PbtIX leaves the site CTGCA|G between the A and G as shown. (As always, the DNA sequence shown is 5'->3'). This leaves a
a. 5' overhang
b. 5' overhang on one end of the cut, and 3' overhang on the other end of the cut
c. blunt end
d. 3' overhang
e. none of the above
Which of the following DNA molecules is complementary to the sequence: 5'-ATTGCGTAC - 3¹?
O a. 5'-TAACGCATG-3'
O b.5'-AUUGCGUAC-3'
O c. 5'- UAACGCAUG - 3'
O d. 5'- GTACGCAAT - 3'
O e. 5'- UTTGCGTUC - 3¹
Convert each of the following 3′-to-5′ DNA base
sequences to 5′-to-3′ DNA base sequences.
a. 3′ ATCG 5′
b. 3′ AATA 5′
c. 3′ CACA 5′
d. 3′ CAAC 5′
Chapter 9 Solutions
Microbiology: An Introduction
Ch. 9 - Compare and contrast the following terms: a. cDNA...Ch. 9 - Differentiate the following terms. Which one is...Ch. 9 - Some commonly used restriction enzymes are listed...Ch. 9 - Suppose you want multiple copies of a gene you...Ch. 9 - Which enzyme makes the smallest fragment...Ch. 9 - Describe a recombinant DNA experiment in two or...Ch. 9 - List at least two examples of the use of rDNA in...Ch. 9 - You are attempting to insert a gene for saltwater...Ch. 9 - How does RNAi silence a gene?Ch. 9 - Prob. 10R
Ch. 9 - Restriction enzymes were first discovered with the...Ch. 9 - The DNA probe, 3-GGCTTA, will hybridize with which...Ch. 9 - Which of the following is the fourth basic step to...Ch. 9 - The following enzymes are used to make cDNA. What...Ch. 9 - If you put a gene in a virus, the next step in...Ch. 9 - You have a small gene that you want replicated by...Ch. 9 - Pieces of human DNA stored in yeast cells. a....Ch. 9 - A population of cells carrying a desired plasmid....Ch. 9 - Self-replicating DNA for transmitting a gene from...Ch. 9 - A gene that hybridizes with mRNA. a. antisense b....Ch. 9 - Design an experiment using vaccinia virus to make...Ch. 9 - Why did the use of DNA polymerase from the...Ch. 9 - The following picture shows bacterial colonies...Ch. 9 - Prob. 1CAECh. 9 - Using the restriction enzyme ECORI, the following...
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