Biology
Biology
12th Edition
ISBN: 9780078024269
Author: Sylvia Mader, Michael Windelspecht
Publisher: McGraw-Hill Education
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Chapter 9, Problem 8A
Summary Introduction

Introduction:

The chromosome of eukaryotes consists of “a single double helix DNA molecule” which is composed of more than 50% proteins. Some of these proteins play role in the synthesis of DNA and RNA while other is majorly histones that play significant structural role.

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Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
a) Calculating Transformation Efficiency For the +DNA/+Amp/+IPTG plate, record the following:  Number of transformants (colonies): _________________ Nanograms of plasmid DNA added: 50 ng  Final recovery volume: 0.50 mL  Volume plated: 0.25 mL  Transformation efficiency equation: Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL) b) Using the equation above, calculate the transformation efficiency. c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?   b) Predict the growth you would expect to see on each of the following plates:         – DNA ___________________________________________________________ – DNA/+Amp ______________________________________________________ +DNA/+Amp ______________________________________________________ +DNA/+Amp/+IPTG _________________________________________________
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