Lehninger Principles of Biochemistry (Instructor's)
7th Edition
ISBN: 9781319117689
Author: nelson
Publisher: MAC HIGHER
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Chapter 9, Problem 5P
Summary Introduction
To design: A PCR base test for identification of Huntington disease (HD) using blood sample.
Introduction:
PCR is a technique used for amplifying single or less number of DNA fragments to form multiple copies. There are different PCR systems based on the source of DNA such as colony PCR, nested PCR, real time PCR, and many more.
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Chapter 9 Solutions
Lehninger Principles of Biochemistry (Instructor's)
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- Sodium borohydride (NaBH4) is a potent inhibitor of aldolase. It is known to ONLY inhibit theenzyme when it is complexed with substrate. Treatment of the enzyme alone has no effect.What is the mechanism for this inhibition? Please draw out the mechanism and show how it inhibits this.arrow_forwardShow the fate of the proton on the 4-Oxygen molecule of F-1,6-BP. Please include a drawing showing the electron flow that occurs.arrow_forward1. Which one is the major organic product obtained from the following aldol condensation? O NaOH, H₂O heat A B C D Earrow_forward
- An organic chemist ordered the wrong item. She wanted to obtain 1-hydroxy-2-butanone, butinstead ordered 2-hydroxybutyraldehyde. As a good biochemist, show how the organic chemistcould use biological catalysis to make her desired compound. Please show the mechanism by drawing.arrow_forwardShow the fate of the hydrogen on carbon-2 of glucose. Please draw out the structure using curve arrows to show electron flow.arrow_forward3. Which one of the compounds below is the major product formed by the reaction sequence shown here? CH3 + CH3NO2 NaOH H2, Ni ? nitromethane acetophenone OH OH HO HN- u x x x x Ph A HO -NH2 HO H Ph Ph Ph N- H B Ph NH2 D Earrow_forward
- 4. Only ONE of the five compounds below can be prepared by an aldol condensation in which a single carbonyl compound is treated with base. Which one is it? To solve this problem, reverse the aldol condensation that formed each of these molecules to find out what two molecules came together to make the products. The one in which the two molecules are identical is the answer. Ph Ph ཚིག གནས ག ནཱ ཀ ན ཀནཱ A Ph H B Ph Ph H D Ph. Ph Ph E Harrow_forward5. Which one is the major organic product obtained from the following reaction sequence? First, equimolar amounts of cyclopentanone and LDA are mixed at -78°C. Then propionaldehyde (propanal) is added. Addition of aqueous acid completes the process. LDA, -78°C. 1. 2. H₂O* H A B H 0 D H H Earrow_forward2. Which one is the major organic product obtained from the following reaction? NaOH, H₂O heat A B C D Earrow_forward
- CH3CH2CHO + propanal PhCH2CHO 2-phenylacetaldehyde mixture of four products NaOH 10. In the crossed aldol reaction of propanal and 2-phenylacetaldehyde shown above, a mixture of four products will be formed. Which ONE of the compounds below will NOT be formed in this crossed aldol reaction? OH Ph A H OH OH Ph H B OH OH H H H Ph Ph C Ph D Earrow_forwardAn organic chemist ordered the wrong item. She wanted to obtain 1-hydroxy-2-butanone, butinstead ordered 2-hydroxybutyraldehyde. As a good biochemist, show how the organic chemistcould use biological catalysis to make her desired compound.arrow_forwardPredict the products of aldolase catalyzing the reaction with acetone and (S)-3-hydroxybutyraldehyde.arrow_forward
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