Chapter 9, Problem 3P

(a)

Summary Introduction

To determine: The other types of recombinant plasmids found among transformed bacteria that are tetracycline resistant and how can they be distinguished.

Introduction:

A plasmid is a small, circular, extra chromosomal DNA. They are naturally occurring entities of bacterial cells, but sometimes they are also seen in some eukaryotic cells. Often, the genes present within the plasmids provide bacteria with certain genetic advantages that include antibiotic resistance. Cell has its own plasmid that naturally provides them resistance towards some antibiotics.

(b)

Summary Introduction

To determine: The different patterns of cloned DNA at each lane.

Introduction:

A plasmid is a small, circular, extra chromosomal DNA. They are naturally occurring entities of bacterial cells, but sometimes they are also seen in some eukaryotic cells. Restriction enzymes are also known as molecular scissors. The restriction enzymes can produce blunt ends as well as sticky ends. In molecular genetics, type II restriction enzymes preparing sticky ends are preferred as the site of recognition and site of cleavage is the same for Type II restriction enzyme unlike the other two.