Biology Now (Core Edition)
Biology Now (Core Edition)
15th Edition
ISBN: 9780393644180
Author: Anne Houtman, Megan Scudellari, Cindy Malone, Anu Singh-Cundy
Publisher: W. W. Norton & Company
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Chapter 9, Problem 1TQ
Summary Introduction

Nucleotides are the building blocks of deoxyribonucleic acid (DNA). DNA is a genetic material and it transfers genetic information from one generation to another. In replication, two identical copies of DNA molecules are produced from the single DNA. The synthesis of ribonucleic acid (RNA) from a specific segment of deoxyribonucleic acid (DNA) is known as transcription. The synthesis of the protein from the mRNA sequence in the cytoplasm is known as translation. Replication, transcription, and translation are the central dogma of biology.

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Answer to Problem 1TQ

The following terms are linked with their appropriate definitions and they are given below:

Biology Now (Core Edition), Chapter 9, Problem 1TQ

Explanation of Solution

Gene expression: Genes are transcribed into RNA molecules (for example, mRNA) and then translated into proteins through gene expression. In transcription, the mRNA is produced from the protein-coding gene and that mRNA is transported to the cytoplasm of the cell. Ribosomes convert the mRNA molecule into protein in the cytoplasm.

Gene regulation: The changing of the expression of genes in response to internal and external signal permits the organism to adapt to its surroundings through the synthesis of various proteins as required.

Transcription: The synthesis of RNA (for example, mRNA) from the DNA template by RNA polymerase is known as transcription. In transcription, the information present in the template DNA is converted into RNA.

Translation: The conversion of information present in the mRNA sequence into protein by ribosome is known as translation. Ribosomes synthesize protein according to the mRNA base sequence by linking amino acids. Proteins are composed of amino acids and they plays an important role in the structure and function of the cells.

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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?   The cell membranes are more resistant than normal.   An isotonic solution had been added instead of water.   A solution of 0.1 M NaCl had been added instead of water.   Not enough water had been added to the red blood cell pellet.   The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?   The cell membranes are more resistant than normal.   An isotonic solution had been added instead of water.   A solution of 0.1 M NaCl had been added instead of water.   Not enough water had been added to the red blood cell pellet.   The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin
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QCE Biology: Introduction to Gene Expression; Author: Atomi;https://www.youtube.com/watch?v=a7hydUtCIJk;License: Standard YouTube License, CC-BY