Microbiology with Diseases by Taxonomy (5th Edition)
5th Edition
ISBN: 9780134019192
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
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Chapter 9, Problem 10MC
Summary Introduction
Introduction:
Antimicrobial agents are the substances that are used for the removal of microorganism. Radiation is the emission or transmission of energy in the form of waves and particles through a medium.
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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin
Chapter 9 Solutions
Microbiology with Diseases by Taxonomy (5th Edition)
Ch. 9 - In practical terms in everyday use, which of the...Ch. 9 - Prob. 2MCCh. 9 - Prob. 3MCCh. 9 - Prob. 4MCCh. 9 - Prob. 5MCCh. 9 - The endospores or which organism can be used as a...Ch. 9 - A company that manufactures an antimicrobial...Ch. 9 - Prob. 8MCCh. 9 - Prob. 9MCCh. 9 - Prob. 10MC
Ch. 9 - Prob. 11MCCh. 9 - Which of the following adjectives best describes a...Ch. 9 - Biosafety Level 3 includes ________. a. double...Ch. 9 - Prob. 14MCCh. 9 - Prob. 15MCCh. 9 - Prob. 16MCCh. 9 - Prob. 17MCCh. 9 - Prob. 18MCCh. 9 - Prob. 19MCCh. 9 - Prob. 20MCCh. 9 - Prob. 1SACh. 9 - Compare and contrast four tests that have been...Ch. 9 - Prob. 3SACh. 9 - Prob. 4SACh. 9 - Prob. 5SACh. 9 - Describe five physical methods of microbial...Ch. 9 - Prob. 7SACh. 9 - Prob. 8SACh. 9 - Prob. 9SACh. 9 - Compare and contrast the action of alcohols,...Ch. 9 - Prob. 11SACh. 9 - Why are alcohols more effective in a 70% solution...Ch. 9 - Contrast the structures and actions of soaps and...Ch. 9 - What are some advantages and disadvantages of...Ch. 9 - Prob. 15SACh. 9 - Calculate the decimal reduction time (D) for the...Ch. 9 - Prob. 2VICh. 9 - Prob. 1CTCh. 9 - Is desiccation the only antimicrobial effect...Ch. 9 - Prob. 3CTCh. 9 - Prob. 4CTCh. 9 - Over 1000 people developed severe diarrhea, and at...Ch. 9 - An over-the-counter medicated foot powder contains...Ch. 9 - Prob. 7CTCh. 9 - Prob. 8CTCh. 9 - Explain why quaternary ammonium compounds are not...Ch. 9 - Prob. 10CTCh. 9 - Would you expect Gram-negative bacteria or...Ch. 9 - Where should you place a sterilization indicator...Ch. 9 - Why is liquid water necessary for microbial...Ch. 9 - Prob. 14CTCh. 9 - During the fall 2001 bioterrorist attack in which...Ch. 9 - Prob. 16CTCh. 9 - Prob. 17CTCh. 9 - Prob. 1CMCh. 9 - Why does milk eventually go bad despite being...Ch. 9 - Why are BSL-4 suits pressurized? Why not just wear...Ch. 9 - Prob. 3TMWCh. 9 - Prob. 4TMWCh. 9 - Prob. 1EDCS
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- With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobinarrow_forwardWhich ONE of the following is FALSE regarding haemoglobin? It has two alpha subunits and two beta subunits. The subunits are joined by disulphide bonds. Each subunit covalently binds a haem group. Conformational change in one subunit can be transmitted to another. There are many variant ("mutant") forms of haemoglobin that are not harmful.arrow_forwardWhich ONE of the following is FALSE regarding haemoglobin? It has two alpha subunits and two beta subunits. The subunits are joined by disulphide bonds. Each subunit covalently binds a haem group. Conformational change in one subunit can be transmitted to another. There are many variant ("mutant") forms of haemoglobin that are not harmful.arrow_forward
- During a routine medical check up of a healthy man it was found that his haematocrit value was highly unusual – value of 60%. What one of the options below is the most likely reason? He will have a diet high in iron. He is likely to be suffering from anaemia. He lives at high altitude. He has recently recovered from an accident where he lost a lot of blood. He has a very large body size.arrow_forwardExplain what age of culture is most likely to produce an endospore?arrow_forwardExplain why hot temperatures greater than 45 degrees celsius would not initiate the sporulation process in endospores?arrow_forward
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