Macroscale and Microscale Organic Experiments
7th Edition
ISBN: 9781305577190
Author: Kenneth L. Williamson, Katherine M. Masters
Publisher: Brooks Cole
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Question
Chapter 8, Problem 8Q
Interpretation Introduction
Interpretation:
The necessity of conducting TLC in a closed chamber and saturation of interior with vapor of the solvent should be determined.
Concept introduction:
Thin layer chromatography works on the basic principle of separation of the constituents of the sample based on its interaction with the mobile and the stationary phase. The sample depending on the extent of its polarity gets separated on the stationary phase under the influence of the mobile phase.
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2. Consider the following example GC Chromatogram from a publication about aggression among
Mediterranean Acrobat Ants (C. scutellaris). This particular sample was generated by rinsing
hydrocarbons from ant exoskeletons. From the chromatogram, what is the minimum number of
unique compunds present in the sample? What data led you to that conclusion?
(Don't overthink it)
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Counts (Abundance)
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"Example of a Gas chromatography-Mass spectrography (GS-MS) chromatogram of hydrocarbon molecules in C. scutellaris" by Filippo
Frizzi, et.al, in PLoS One is licensed under CC BY 4.0
Explain why TLC cannot be used for some volatile low molecular weight substances such as
toluene.
Differentiate normal phase chromatography from reverse phase chromatography. From which of the two governs the video demonstration in TLC and paper chromatography? How do you say so?
Chapter 8 Solutions
Macroscale and Microscale Organic Experiments
Ch. 8 - Prob. 1QCh. 8 - Prob. 2QCh. 8 - What problem will ensue if the level of the...Ch. 8 - Prob. 4QCh. 8 - Prob. 5QCh. 8 - Arrange the following in order of increasing Rf...Ch. 8 - What will be the result of applying too much...Ch. 8 - Prob. 8QCh. 8 - Prob. 9QCh. 8 - A TLC plate showed two spots with Rf values of...
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- Why is GSC not used nearly as extensively as GLC?arrow_forwardUse a suitable model to explain how separation and identification of a mixture of organic compounds can be achieved with a thin layer chromatographic (TLC) technique.arrow_forward4.Discussed the Purposed of the Golden rules in the practice of Forensic Chrmistry.arrow_forward
- Aside from TLC and paper chromatography, what are the other types of chromatography. Discuss their principles, applications and limitations.arrow_forwardCan you help me answer these questions?arrow_forwardContrast the physical appearance of the crude material before the recrystallization and that of the material isolated after recrystallization.arrow_forward
- In normal-phase HPLC chromatography, the stationary phase is polar and the mobile phase is nonpolar. In the reversed phase we have just the opposite; the stationary phase is nonpolar and the mobile phase is polar. Describe molecules in which move faster or slower in each case.arrow_forwardWhy do we not need "spitless" injection to measure the toluene content of gasoline using gas chromatography?arrow_forwardAre the ammonia distillation and the AAS a macroanalysis or a microanalysis? In which analysis is there a greater chance of percentage deviations in the result?arrow_forward
- 3. What is particulate gravimetry? When do we use this technique? 4. What are the conditions for a successful gravimetric analysis. 5. Define nucleation. What conditions are important for this stage.arrow_forwardBoth melting points and TLC rely on differences in non covalent forces. Explain the role of these non-covalent interactions in the final answer obtained (melting point and Rf value).arrow_forward6. Discuss briefly why the following TLC experiments were not performed properly A B 7. Arrange the following compounds in order of increasing the polarity: CH3(CH2),0CH3, CH;(CH2)20H, CH;(CH2);CO2H, CH;(CH2)«CH3, CH3(CH2);COHarrow_forward
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