Macroscale and Microscale Organic Experiments
7th Edition
ISBN: 9781305577190
Author: Kenneth L. Williamson, Katherine M. Masters
Publisher: Brooks Cole
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Question
Chapter 8, Problem 5Q
Interpretation Introduction
Interpretation:
The expectations should be determined when the TLC plate is allowed to remain the chamber of development for long time, so that the solvent reaches at the top of the plate during analysis of mixture.
Concept introduction:
TLC works on the principle of separation of constituents of a mixture of compounds based on polarity. The polar compounds interact with the stationary phase thus can move to smaller distance from the base. While the non polar compounds tend to move faster as they interact less with the silica gel; therefore, they move far from the base of the plate.
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Chapter 8 Solutions
Macroscale and Microscale Organic Experiments
Ch. 8 - Prob. 1QCh. 8 - Prob. 2QCh. 8 - What problem will ensue if the level of the...Ch. 8 - Prob. 4QCh. 8 - Prob. 5QCh. 8 - Arrange the following in order of increasing Rf...Ch. 8 - What will be the result of applying too much...Ch. 8 - Prob. 8QCh. 8 - Prob. 9QCh. 8 - A TLC plate showed two spots with Rf values of...
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Similar questions
- A standard sample of compound A was placed on a silica TLC plate. After elution in 30/70 ethyl acetate/hexane, the sample showed a single spot at 3.4 cm (relative to the initial point) after the solvent had risen to 7.1 cm (relative to the initial point). A sample which was thought to contain this compound was analyzed by TLC and showed two spots after elution in the same solvent system. The solvent height for this plate was 6.5 cm (relative to the initial point) and the spots were located at 3.6 cm and 5.2 cm (relative to the initial point). Can either of these spots possibly be compound A? Explain. Include a discussion of the R, for each spot.arrow_forwardConsider a sample that is a mixture composed of biphenyl, benzoic acid, and benzyl alcohol. Predict the order of elution of the components in this mixture. Assume that the chromatography uses a silica column, and the solvent system is based on cyclohexane, with an increasing proportion of methylene chloride being added as a function of time.arrow_forwardDifferentiate between isocratic elution and gradient elution. Why is gradient elution sometimes a necessary technique in HPLC? What are the steps involved in gradient elution?arrow_forward
- 2. What potential problem can a student have when an R₁ = 0 is obtained for an unknown sample on a silica TLC plate developed with a hexane mixture. How can this problem be avoided involving another TLC experiment?arrow_forwardAt the completion of the experiment, a student measures the solvent front to be 55 mm from the baseline. If a component in the sample being analyzed generates a spot whose diameter spans from 34 to 36 mm from the baseline, calculate the Rf value for that component.arrow_forwardÑ1arrow_forward
- In color chromatography of plant pigments, what complications would a dried-out column (solvent level is below the top of the silica) introduce to the elution and isolation of pigments?arrow_forwardAn orange compound, dissolved in dichloromethane, is added to a chromatogra- phy column. The elution is begun using hexane as the solvent. After 6 L of solvent was passed through the column, the orange band had still not traveled down the column appreciably. What should be done to make this experiment work better.arrow_forwardIf the TLC plates had been developed in a more polar solvent, would retention factors on the whole be larger, smaller, or unchanged? Briefly (1 - 2 sentences) explain your reasoningarrow_forward
- In the eluent generator used in the ion chromatograph, the [KOH] generated depends on the applied current and the flow rate. How can the [KOH] be increased at a given flow rate?arrow_forwardExplain how it is that SPE cartridges & disks allow analysts to more efficiently pre- concentrate analytes from samples where they exist at low concentrations.arrow_forwardIn reversed phase HPLC, how will the retention time of a component of a mixture vary with the polarity of its molecules? Explain your answerarrow_forward
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