Concept explainers
To explain: The reason why the treatment of trypsin, collagenase, and EDTA is necessary for the isolation of cell.
Introduction: Trypsin is a serine protease from the protease family. It is mainly found in the
To explain: Why the treatment of trypsin, collagenase, and EDTA in isolation of cell does not kill the cells.
Introduction: Trypsin is a serine protease from the protease family. Collagenase enzyme helps in the fragmentation of the peptide bond in collagen. Ethylenediaminetetraacetic acid (EDTA) is mainly used to disintegrate lime scale.
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Molecular Biology of the Cell (Sixth Edition)
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