Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
6th Edition
ISBN: 9780815344322
Author: Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher: W. W. Norton & Company
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Chapter 8, Problem 7P
Summary Introduction

To explain: The reason why the treatment of trypsin, collagenase, and EDTA is necessary for the isolation of cell.

Introduction: Trypsin is a serine protease from the protease family. It is mainly found in the digestive system of several vertebrates. In the vertebrate digestive system, trypsin helps in the hydrolysis of protein. Collagenase is an enzyme that helps in the fragmentation of the peptide bond in collagen. Ethylenediaminetetraacetic acid (EDTA) is an aminopolycarboxylic acid and is a colorless water soluble solid mainly used to disintegrate lime scale.

Summary Introduction

To explain: Why the treatment of trypsin, collagenase, and EDTA in isolation of cell does not kill the cells.

Introduction: Trypsin is a serine protease from the protease family. Collagenase enzyme helps in the fragmentation of the peptide bond in collagen. Ethylenediaminetetraacetic acid (EDTA) is mainly used to disintegrate lime scale.

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