Microbiology with Diseases by Body System (4th Edition)
Microbiology with Diseases by Body System (4th Edition)
4th Edition
ISBN: 9780321918550
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
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Chapter 8, Problem 7CT
Summary Introduction

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Antisense RNA used to terminate translation of TACAATCGCATTGAA sequence.

Introduction:

In translation, m-RNA is converted into amino acid sequences, resulting in protein synthesis. Three important components are involved in translation are m-RNA, t-RNA, and the ribosome. Protein synthesis occurs in the cytoplasm of the cell. The mRNA sequence encoded by the genetic material is translated into a specific protein. The t-RNA binds to free amino acids and transfer them to the ribosome and the amino acids are added to the growing chain of the protein sequence. The ribosome reads m-RNA and synthesizes protein based on codons present in the m-RNA sequence. The ribosome binds to the anticodon of particular tRNA according to m-RNA sequence and assembles amino acids corresponding to mRNA codons. Three nucleotides of m-RNA sequence code for a single amino acid of the protein sequence is known as a codon. The twenty amino acids are represented by a different pattern of four nitrogenous bases is called as the genetic code. Sixty four (64) codons (different pattern of three nucleotides) encode 20 amino acids. Protein synthesis occurs in three steps which are initiation, elongation, and termination. AUG is the start codon, which initiates protein synthesis from m-RNA sequence, whereas, UAG, UGA, and UAA are stop codons which terminate protein synthesis.

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After you feel comfortable with your counting method and identifying cells in the various stages of mitosis, use the four images below of whitefish blastula to count the cells in each stage until you reach 100 total cells, recording your data below in Data Table 1. (You may not need to use all four images. Stop counting when you reach 100 total cells.) After totaling the cells in each stage, calculate the percent of cells in each stage. (Divide total of stage by overall total of 100 and then multiply by 100 to obtain percentage.)   Data Table 1Stage    Totals    PercentInterphase        Mitosis:        Prophase        Metaphase        Anaphase        Telophase        Cytokinesis        Totals    100    100% To find the length of time whitefish blastula cells spend in each stage, multiply the percent (recorded as a decimal, in other words take the percent number and divide by 100) by 24 hours. (Example: If percent is 20%, then Time in Hours = .2 * 24 = 4.8) Record your data in Data…
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