Essential Cell Biology (fifth Edition)
Essential Cell Biology (fifth Edition)
5th Edition
ISBN: 9780393680362
Author: ALBERTS, Bruce, Hopkin, Karen, Johnson -
Publisher: W. W. Norton & Company
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Chapter 8, Problem 6Q
Summary Introduction

To explain: The expected results of the given experiment.

Concept introduction:

Transcription process marks the beginning of gene expression. Transcription factors are the proteins that function in transcribing DNA to mRNA. Enhancers are distal control elements located away from the transcribing gene. The control elements of enhancers are short DNA nucleotide sequences. They regulate the expression of specific target genes by binding to the specific transcription factors. These may be activators (activate transcription) or repressors (repress transcription).

Given:

The experiment is designed in Professor Q’s laboratory to determine how far an enhancer be moved from the promoter region of the straight spine gene and still initiate transcription. In order to determine this, the numbers of nucleotide pairs are varied between promoter and enhancer sites. The graph shows the data after measuring the production of straight spine mRNA.

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Question #3: In the KeyGene paper, the authors state that it would be useful if pollen from an apomict would transmit apomixis-inducing genes to the female in the cross (assuming the pollen is viable). Assuming there was just one gene conferring gametophytic obligate apomixis, and that the two parents are inbreds, what would be the consequences of such a cross if: a) The apomixis was a dominant trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the parental cross. b) The apomixis was a recessive trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the…
Question #5: Assume that two genes are identified that confer gametophytic facultative apomixis in soybean. The genes show independent assortment. Recessive alleles at both loci are required for the facultative apomixis. Facultative apomixis is triggered when the temperature at pollination is above 20 degrees C. At temperatures below 20 degrees C, all reproduction is sexual, independent of genotype. A facultative apomict male, capable of producing viable pollen, was crossed with a sexually reproducing female. Assuming the parents are completely inbred, what are the predicted phenotypic ratios (apomict: non-apomict) for the F1, F2, and DH (F1-derived) generations at each of the following temperatures*: a) 15°C? b) 25°C? *for full credit, show crosses and genotypes where appropriate. Remember to position the female first (left side) in the cross. Type your answer here:
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QCE Biology: Introduction to Gene Expression; Author: Atomi;https://www.youtube.com/watch?v=a7hydUtCIJk;License: Standard YouTube License, CC-BY