Campbell Biology in Focus
3rd Edition
ISBN: 9780134710679
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Rebecca Orr
Publisher: PEARSON
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Chapter 8, Problem 4TYU
Which of the following statements is a correct distinction between autotrophs and heterotrophs?
- A. Autotrophs, but not heterotrophs, can nourish themselves beginning with CO2 and Other nutrients that are inorganic.
- B. Only heterotrophs require chemical compounds from the environment.
- C.
Cellular respiration is unique to heterotrophs. - D. Only heterotrophs have mitochondria.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the
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oxygen is terminal electron acceptor
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Chapter 8 Solutions
Campbell Biology in Focus
Ch. 8.1 - How do the CO2 molecules used in photosynthesis...Ch. 8.1 - How did the use of an oxygen isotope help...Ch. 8.1 - WHAT IF? The Calvin cycle requires ATP and NADPH,...Ch. 8.2 - What color of light is least effective in driving...Ch. 8.2 - In the light reactions, what is the initial...Ch. 8.2 - Prob. 3CCCh. 8.3 - MAKE CONNECTIONS How are the large numbers of ATP...Ch. 8.3 - WHAT IF? Explain why a poison that inhibits an...Ch. 8.3 - Prob. 3CCCh. 8.4 - Prob. 1CC
Ch. 8 - The light reactions of photosynthesis supply the...Ch. 8 - Which of the following sequences correctly...Ch. 8 - How is photosynthesis similar in C4, plants and...Ch. 8 - Which of the following statements is a correct...Ch. 8 - Which of the following does not occur during the...Ch. 8 - In mechanism, photophosphorylation is most similar...Ch. 8 - Prob. 7TYUCh. 8 - To synthesize one glucose molecule, the Calvin...Ch. 8 - SCIENCE, TECHNOLOGY, AND SOCIETY Scientific...Ch. 8 - DRAW IT The following diagram represents an...Ch. 8 - Prob. 11TYUCh. 8 - Prob. 12TYUCh. 8 - FOCUS ON ENERGY AND MATIER Life is solar powered....Ch. 8 - SYNTHESIZE YOUR KNOWLEDGE Watermelon snow in...
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