
A.
To determine: What would happen if bacteria containing a phage in prophage state are irradiated with UV light and c1 protein is degraded.
Introduction: When bacteriophages infect a host cell, their DNA follows two fates in the form of lytic and lysogenic cycles. Lytic cycle refers to generation of new phages and lysis of a cell. Lysogeny also known as prophage stage refers to integration of phage in the host’s genome where it replicates with host DNA over many generations. These are controlled by c1 and Cro transcription regulators encoded by virus.
B.
To determine: Whether the change due to UV irradiation can be reversed when UV light is switched off.
Introduction: When bacteriophages infect a host cell, their DNA follows two fates in the form of lytic and lysogenic cycles. Lytic cycle refers to generation of new phages and lysis of a cell. Lysogeny also known as prophage stage refers to integration of phage in the host’s genome where it replicates with host DNA over many generations. These are controlled by c1 and Cro transcription regulators encoded by virus.
C.
To determine: Why this response to UV light might have evolved.
Introduction: When bacteriophages infect a host cell, their DNA follows two fates in the form of lytic and lysogenic cycles. Lytic cycle refers to generation of new phages and lysis of a cell. Lysogeny also known as prophage stage refers to integration of phage in the host’s genome where it replicates with host DNA over many generations. These are controlled by c1 and Cro transcription regulators encoded by virus.

Want to see the full answer?
Check out a sample textbook solution
- Biology How many grams of sucrose would you add to 100mL of water to make a 100 mL of 5% (w/v) sucrosesolution?arrow_forwardWhich marker does this DNA 5ʹ AATTGGCAATTGGCAATTGGCAATTGGCAATTGGCAATTGGCAATTGGC 3ʹ show?arrow_forwardThe Z value of LOD for two genes is 4, what does it mean for linkage and inheritance?arrow_forward
- Biology How will you make a 50-ul reaction mixture with 2uM primer DNA using 10 uM primer DNA stocksolution and water?arrow_forwardBiology You’re going to make 1% (w/v) agarose gel in 0.5XTBE buffer 100 ml. How much agarose are you goingto add to 100 ml of buffer? The volume of agaroseis negligible.arrow_forwardBiology How will you make a 50-ul reaction mixture with0.2 mM dNTP using 2-mM dNTP stock solution andwater?arrow_forward
- Biology What is 200 pmole/uL in Molar concentration?arrow_forwardBiology How will you make a 50-ul reaction mixture with 1Xreaction buffer in it using water and 5X buffer stocksolution?arrow_forwardBiology How would you make 200 uL of 10 pmole/uLprimer DNA solution using the 200 pmole/uLprimer DNA stock solution and distilled water?arrow_forward
- Biology Now you have the 5 M of NaCl stock solution. Howwould you make one liter of 100 mM NaCl solutionusing the 5 M of NaCl solution and distilled water?arrow_forwardDevelopmental Biology Lab Question How to make one liter of 5 M NaCl stock solution?The molar weight of NaCl is 58.44 g/mol.(Molecular weight is 58.44 Dalton or amu).arrow_forwardDevelopmental Biology What is the definition of 1 M? (in the context of stock and dilutions)arrow_forward
- Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSONBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStaxAnatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
- Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & CompanyLaboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education





