Microbiology: An Introduction (13th Edition)
Microbiology: An Introduction (13th Edition)
13th Edition
ISBN: 9780134605180
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber, Warner Bair
Publisher: PEARSON
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Chapter 8, Problem 3A

Pseudomonas has a plasmid containing the mer operon, which includes the gene for mercuric reductase. This enzyme catalyzes the reduction of the mercuric ion Hg21 to the uncharged form of mercury, Hg0. Hg21 is quite toxic to cells; Hg0 is not.

  1. a. What do you suppose is the inducer for this operon?
  2. b. The protein encoded by one of the mer genes binds Hg21 in the periplasm and brings it into the cell. Why would a cell bring in a toxin?
  3. c. What is the value of the mer operon to Pseudomonas?
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Linkage in common bean Alleles at the P locus control seed color. Plants which are pp have white seeds, white flowers and no pigment in vegetative parts. Plants which are P_ have black seeds, purple flowers and may have varying degrees of pigment on stems and leaves. Seed color can be assessed, visually, based on if the seed is white or not white A gene for mold resistance has been reported and we want to determine its inheritance and whether it is linked to P. For the purposes of this exercise, we will assume that resistance is controlled by a single locus M, and M_ plants are resistant and mm plants are susceptible.  Resistance can be measured, under greenhouse conditions, 2 weeks after planting, by injecting each seedling with a spore suspension. After two weeks, the seedlings can be rated as resistant or susceptible, based on whether or not tissue is actively sporulating. For this exercise we will use seed and data from the F10 generation of a recombinant inbred population…

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Microbiology: An Introduction (13th Edition)

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