Chapter 8, Problem 18P

Summary Introduction

To determine: The products of partial digestion by snake venom phosphodiesterase of an oligonucleotide with the sequence $\left({\text{5}}^{\prime }\right)\text{GCGCCAUUGC}\left({\text{3}}^{\prime }\right)-\text{OH}$.

Introduction:

An enzyme called exonuclease cleaves ends of nucleotides of a polynucleotide strand. Snake venom phosphodiesterase is responsible for hydrolyzing nucleotides from ${\text{3}}^{\prime }$ end. Snake venom phosphodiesterase is used in determination of nucleic acid sequences before any sequencing techniques were developed.