Four introns labeled A to D are present in eukaryotic gene ( Gen-100 ). Consider that Gen-100 has been isolated and the DNA of the gene ( Gen-100 ) has been denatured. Then it is mixed with polyadenylated mRNAfrom the gene. a. The R-loop structure, seen with electron microscopy is to be illustrated. b. The introns are to be labelled. c. Single stranded or double stranded, intron region is to be determined. Introduction: DNA contains coding and non-coding regions called exons and introns respectively. During post-transcriptional modifications of eukaryotic DNA, the introns are spliced out and mature mRNA is synthesized containing only exons. R-looping was first discovered and described in 1976 by Richard. J. Roberts and Philip A. Sharp. R-loop is a three-stranded structure. It is composed of a hybrid of RNA: DNA and the single-stranded non-template DNA. The R-loops are created in the laboratory by hybridization of mature mRNA and double-stranded DNA. The intronicregion of DNA gets spliced out from the mRNA and forms single-stranded structure as they cannot hybridize with the dsDNA.The spliced intronic regions will form the loops between the exons. These R-loops are observed by electron microscopy.

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Chapter 8, Problem 15P

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Four introns labeled A to D are present in eukaryotic gene (Gen-100). Consider that Gen-100 has been isolated and the DNA of the gene (Gen-100) has been denatured. Then it is mixed with polyadenylated mRNAfrom the gene.

a. The R-loop structure, seen with electron microscopy is to be illustrated.

b. The introns are to be labelled.

c. Single stranded or double stranded, intron region is to be determined.

Introduction:

DNA contains coding and non-coding regions called exons and introns respectively. During post-transcriptional modifications of eukaryotic DNA, the introns are spliced out and mature mRNA is synthesized containing only exons. R-looping was first discovered and described in 1976 by Richard. J. Roberts and Philip A. Sharp. R-loop is a three-stranded structure. It is composed of a hybrid of RNA: DNA and the single-stranded non-template DNA. The R-loops are created in the laboratory by hybridization of mature mRNA and double-stranded DNA. The intronicregion of DNA gets spliced out from the mRNA and forms single-stranded structure as they cannot hybridize with the dsDNA.The spliced intronic regions will form the loops between the exons. These R-loops are observed by electron microscopy.

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